PMID: 375051Mar 13, 1979Paper

Evaluation of a modified dye pour-plate auxanographic method for the rapid identification of clinically significant yeasts. Comparison with two commercial systems

Medical Microbiology and Immunology
L H WeymannS M Qadri

Abstract

A modified dye pour-plate auxanographic (DPPA) method for the presumptive identification of medically important yeasts was evaluated, in a comparative study with a conventional procedure, the API 20C clinical yeast system (Analytab Products Inc.), and the Uni-Yeast-Tek (UYT) system. The 174 coded clinical isolates were members of the genera Candida, Cryptococcus, Rhodotorula, Saccharomyces, Torulopsis, and Trichosporon. The identification accuracies with DPPA, API, and UYT were 95%, 93%, and 99% respectively. DPPA and API required more time to inoculate but gave rapid identification profiles. UYT was simple to inoculate and both UYT and DPPA were easy to read. Cost analysis of the three rapid methods demonstrated DPPA to be the most economical making it a feasible alternative for small clinical laboratories as well as large laboratories possessing the facilities to make their own media.

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Citations

May 26, 1978·Medical Microbiology and Immunology·S M Qadri, C W Nichols
Jan 1, 1986·Medical Microbiology and Immunology·S M QadriE G Ramirez
Jan 1, 1981·Critical Reviews in Microbiology·R F D'AmatoE J Bottone
Jan 1, 1987·Critical Reviews in Microbiology·C C Lin, D Y Fung
Sep 1, 1995·Journal of Clinical Pathology·K G DaveyD W Warnock
Apr 24, 2010·Journal of Medical Microbiology·Rajendra J KothavadeM H Panthaki

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