Evaluation of a modified HET-CAM assay as a screening test for eye irritancy

Toxicology in Vitro : an International Journal Published in Association with BIBRA
L GilleronP Vanparys

Abstract

The hen's egg test-chorioallantoic membrane (HET-CAM) assay, an alternative to the Draize eye irritation test, was developed by Luepke and has been improved on by means of a microscopic examination and the use of a test substance applicator (TSA). The TSA is a double teflon ring in which a perlon mesh is locked, and has several advantages over conventional protocols, reducing subjectivity of the method and avoiding the need for rinsing after treatment. It was confirmed by statistical analysis that the HET-CAM-TSA method can reproduce potentialin vivo irritant effects on the conjunctiva. The classification based on thein vitro results was compared with fourin vivo classifications [MAS (maximal average score) with thresholds of 15.0 and 25.0; the Kay and Calandra method; and EC criteria]. Cooper's parameters (specificity, sensitivity and concordance with the Draize test) were calculated according to these fourin vivo classifications. When the most rigorous classification (MAS threshold of 15.0) was taken into account, a sensitivity of 80%, a specificity of 81.3% and a concordance with the Draize test of 80.4% were obtained for this set of 46 compounds.

References

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Citations

Oct 1, 1997·Toxicology in Vitro : an International Journal Published in Association with BIBRA·L GilleronP Vanparys
Feb 1, 1999·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S HaginoY Ohno
Jan 1, 2011·International Journal of Pharmaceutical Investigation·Ritu Mehra GilhotraDina Nath Mishra
Aug 13, 2013·Journal of Pharmacological and Toxicological Methods·G EichenbaumL Lammens
Aug 19, 2007·Journal of Pharmaceutical Sciences·M P Vinardell, M Mitjans
Jan 13, 2006·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M P Vinardell, M Mitjans
Oct 18, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Marijke M M DhondtJean Paul Remon
Jun 2, 2009·International Journal of Toxicology·A S KishoreP Balakrishna Murthy
Oct 1, 2005·Alternatives to Laboratory Animals : ATLA·Chantra EskesValérie Zuang
Aug 29, 2020·Translational Vision Science & Technology·Carolina Nunes da SilvaArmando da Silva Cunha-Jr
Apr 23, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Martín Nicolás RiveroMaría Laura Gutiérrez

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