Evaluation of a Multilocus Sequence Typing (MLST) scheme for Leishmania (Viannia) braziliensis and Leishmania (Viannia) panamensis in Colombia

Parasites & Vectors
Giovanny HerreraJuan David Ramírez

Abstract

Leishmaniases are parasitic vector-borne diseases affecting more than 12 million people in 98 countries. In Colombia, leishmaniasis is widespread and the most common clinical manifestation is cutaneous, mainly caused by L. panamensis and L. braziliensis. Currently, the genetic diversity of these species in Colombia is unknown. To address this, we applied molecular techniques for their characterization, using multilocus sequence typing (MLST) to explore the genetic variability and phylodynamics of the disease. Seven previously described genetic markers were selected highlighting the implementation of a mitochondrial marker. Markers were applied to 163 samples from isolates obtained between 1980 and 2001. The identification of the samples showed an excellent correlation with typing tests previously applied (MLEE, monoclonal antibodies). Isolates of L. braziliensis showed greater genetic diversity than L. panamensis, and a greater number of diploid sequence types (DSTs). In addition, the geographical distribution of DSTs for each species were obtained through georeferencing maps. To our knowldge, this study represents the first description of the genetic variability of L. panamensis in Colombia and South America, and is the first ...Continue Reading

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Citations

Apr 7, 2018·PLoS Neglected Tropical Diseases·Giovanny HerreraJuan David Ramírez
Aug 18, 2020·PLoS Neglected Tropical Diseases·Nathalia PinhoPatricia Cuervo
Jul 12, 2020·Genes·Adriana HigueraJuan David Ramírez
Mar 27, 2020·ELife·Susanne U FranssenJames A Cotton
Nov 13, 2020·Frontiers in Cellular and Infection Microbiology·Luz H PatinoJuan David Ramírez
Oct 4, 2019·Acta Tropica·Juan Jose LauthierMasataka Korenaga
Sep 13, 2020·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Mansoure HosseiniAli Khamesipour
Jul 27, 2021·PLoS Neglected Tropical Diseases·Insaf Bel Hadj AliIkram Guizani
Aug 25, 2021·European Journal of Pharmacology·Diksha KumariKuljit Singh

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Methods Mentioned

BETA
electrophoresis
biopsies
PCR

Software Mentioned

PHASE
ArcGIS10
TriTrypDB
eBURST
MEGA
Excel
MLSTest
DnaSP
MLST
RAxML

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