PMID: 7536231Jan 1, 1995Paper

Evaluation of a multiple peptide assay for typing of antibodies to the hepatitis C virus: relation to genomic typing by the polymerase chain reaction

Journal of Medical Virology
Z X ZhangM Sällberg

Abstract

A panel of 16 type-specific synthetic peptides corresponding to variable antigenic regions within the hepatitis C virus (HCV) core, nonstructural 4 (NS4), and NS5 proteins was synthesised. The peptide panel was used to develop an enzyme immunoassay (EIA) for the detection of antibodies directed to HCV type 1 (genotypes I/1a and II/1b), type 2 (genotypes III/2a and IV/2b), and type 3 (genotype V/3). The peptides corresponded to residues 68-81 of the HCV core (types 1, 2, and 3), residues 1692-1705 and 1710-1728 of HCV NS4 (types 1a, 1b, 2a, 2b, and 3), and residues 2303-2319 of HCV NS5 (types 1a, 1b, 2a, and 2b). The 16-peptide panel was evaluated using human sera from 46 carriers of HCV, which were genotyped in parallel by the polymerase chain reaction (PCR) using primers specific for types I, II, III, IV, and V of HCV core. Of the 46 carriers, 14 (30%) were infected by HCV genotype I, 7 (15%) by genotype II, 16 (35%) by HCV genotype IV, and 6 (13%) by HCV of genotype V. Two carriers had double infections of types I and II, and the HCV strain of one carrier could not be genotyped. Using the serotyping system, 40 (89%) out of the 45 genotyped carriers were found to contain type-specific antibodies corresponding to the genotypes ...Continue Reading

References

May 1, 1992·The Journal of General Virology·S W ChanP Simmonds
Aug 1, 1992·Journal of Clinical Microbiology·M SällbergL O Magnius
Oct 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A MachidaS Mishiro
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·Q L ChooP J Barr
Mar 25, 1991·FEBS Letters·N KatoK Shimotohno
Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J BukhR H Miller

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Citations

Nov 5, 1997·The Veterinary Quarterly·R H MeloenJ P Langeveld
Aug 1, 1996·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Giuseppe Vittorio Luigi De SocioGiuliano Stagni
Jul 10, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M ChenD R Milich
Dec 17, 1997·Journal of Clinical Microbiology·J A NevilleP Simmonds

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