Evaluation of a multiplex panel for the diagnosis of acute infectious diarrhea in immunocompromised hematologic patients

PloS One
Izaskun Alejo-CanchoMª Ángeles Marcos

Abstract

Diarrhea is a frequent complication in hematologic patients, being an infectious cause frequently suspected. Rapid and accurate detection of gastrointestinal pathogens is vital in immunocompromised hosts. The aim of this study was to compare routine diagnostic methods versus a multiplex polymerase chain reaction (PCR) assay for the diagnosis of infectious diarrhea in immunocompromised hematologic patients. We conducted a prospective observational study from March 2015 to January 2016 to compare conventional methods for the diagnosis of infectious diarrhea with FIlmArray GI Panel (BioFire-bioMérieux, France). Samples from adult immunocompromised hematologic patients with acute diarrhea were collected. In cases with discordant results, a second multiplex assay was performed (Allplex, Seegene, Korea). The result was considered positive or negative when the same result was obtained by at least two of the methods. A total of 95 samples were obtained from 95 patients (median age of 52 years (46-64)). Sixty-one (64%) episodes were hospital-acquired and 34 (36%) were community-acquired diarrhea. Twenty-five (26%) patients had a positive microbiological result, being Clostridium difficile the most frequent pathogen, followed by Campylob...Continue Reading

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Citations

Nov 9, 2018·Indian Journal of Gastroenterology : Official Journal of the Indian Society of Gastroenterology·Ujjala Ghoshal, Nidhi Tejan
Jul 30, 2019·Current Opinion in Infectious Diseases·Phillip P SantoiemmaMichael P Angarone
Mar 28, 2018·Journal of Clinical Microbiology·Stacy G BealKenneth H Rand
Sep 24, 2018·Indian Journal of Gastroenterology : Official Journal of the Indian Society of Gastroenterology·Balavinoth RamakrishnanV Ramasubramanian
Nov 28, 2019·Current Infectious Disease Reports·Brittany A YoungCarlos A Gomez
Nov 7, 2019·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jennifer L Saullo, Christopher R Polage
Mar 5, 2021·Infectious Diseases and Therapy·Tamar BergerMoshe Yeshurun
May 25, 2021·Gastroenterology Clinics of North America·Jeffery L Meier
Jun 16, 2021·Journal of Gastroenterology and Hepatology·Readon TehKewin Tien Ho Siah

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Methods Mentioned

BETA
PCR
PCRs

Software Mentioned

Allplex
SPSS
Epidat

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