Evaluation of a multitest system for rapid identification of Salmonella and Shigella

American Journal of Clinical Pathology
W M Gooch

Abstract

The ability of Micro-ID, a multitest system for rapid (four hour) identification of Enterobacteriaceae, to identify Salmonella and Shigella was evaluated. Micro-ID, API 20E and a battery of tubed media consisting of triple sugar-iron agar, Christensen's urea agar, and Moeller's lysine decarboxylase medium were used to study 516 lactose nonfermenting strains of Enterobacteriaceae isolated from 500 consecutive pediatric stool specimens. Fifty-six of the isolates were Salmonella, and 21 were Shigella. Micro-ID correctly identified all isolates of Shigella and all but one isolate of Salmonella, whereas the conventional screening media failed to detect seven isolates of Salmonella and two isolates of Shigella. The false-positive rates were 1% and 20% for Micro-ID and the conventional battery, respectively. Use of Micro-ID as a substitute for conventional screening media for lactose nonfermenting stool isolates provides reliable presumptive idenfication of Salmonella and Shigella within four hours.

Citations

May 1, 1982·Journal of Clinical Microbiology·W M Gooch, G A Hill

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.