Evaluation of a new screening assay for HTLV-1 and -2 antibodies for large-scale use

Journal of Medical Virology
Kerstin MalmSören Andersson

Abstract

Laboratory testing for Human T-lymphotropic Virus type 1 and 2 (HTLV-1 and -2) infections has become routine in blood transfusion, tissue transplantation and clinical diagnoses in many countries worldwide. Screening is usually based on the detection of antibodies to HTLV-1 and/ or -2. The number of commercially available assays is limited, and among them, ELISA tests based on microtiter format are most commonly used. Recently, the new rHTLV-I/II assay (Abbott Laboratories, Abbott Park, IL) was released; this assay was developed for an automatic large-scale screening platform. This assay was evaluated using pre-characterized serum panels and routine samples from the clinical laboratory. The sensitivity was 100% for HTLV-1 and -2 (99/99 and 42/42, respectively, including one sample that was dually reactive, HTLV-1 + 2). To test assay specificity, panels of blood donor sera, specimens from patients with autoimmune diseases and some viral infections were used. False-reactive samples from previous HTLV diagnoses were also included. With these panels, the specificity was 99.4% (619/623). However, the four false-reactive samples all belonged to the group of samples that were previously considered as false-reactive for HTLV-antibodies....Continue Reading

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Citations

Oct 13, 2012·Transplantation·Matthew J ArmstrongJames M Neuberger
Jul 19, 2012·Scandinavian Journal of Infectious Diseases·Kerstin MalmSören Andersson
Apr 7, 2015·Seminars in Oncology·Brian J MorrisonDenise Whitby
Feb 11, 2020·Cancer Investigation·Sharel Mendonca BryneMuhammed Thahir
Sep 21, 2018·Journal of Clinical Microbiology·Vanessa da Silva BritoBernardo Galvão-Castro

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