Evaluation of a new Sebia kit for analysis of hemoglobin fractions and variants on the Capillarys system

Clinical Chemistry and Laboratory Medicine : CCLM
Amal LouahabiDiane Maisin

Abstract

We evaluated the analytical performances of the new Sebia kit for quantification of hemoglobin fractions (HbA, HbF and HbA2) and structural hemoglobin variants on the Capillarys system. This automated capillary zone electrophoresis method uses an alkaline buffer with silica capillaries and spectrophotometric detection. Specimen stability was evaluated during 1 month. The reproducibility of migration and the imprecision of quantification were also investigated. Comparison with the Beckman P/ACE system was performed on 202 samples. A total of 131 subjects without any hematological abnormality were analyzed to establish the HbA2 reference ranges based on our local population. Quantification of the Hb fractions and variants exhibited excellent stability for 4 weeks of storage at 4 degrees C, with CVs < 0.3%. The imprecision of the migration normalized to that of HbA2 for all hemoglobins tested (fractions and variants) was low, with a CV of < 2.5%. At physiological and pathological levels, total imprecision ranged from 1.9% to 4.6% for HbA2, from 0.6% to 9.7% for HbF, and from 0.6% to 1% for HbS. Statistical analysis revealed a small proportional negative bias for HbA2 (-8.6%). Small systematic bias (-0.2%) and proportional bias (-2...Continue Reading

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Citations

Dec 29, 2010·Annals of Hematology·Thongperm MunkongdeeSuthat Fucharoen
Nov 17, 2009·Clinical Chemistry and Laboratory Medicine : CCLM·Gilles RenomJean-Marc Périni
Nov 20, 2015·Translational Research : the Journal of Laboratory and Clinical Medicine·Annamaria AprileFerruccio Ceriotti
Mar 21, 2012·American Journal of Clinical Pathology·David F KerenDeborah Hedstrom
Oct 23, 2008·Translational Research : the Journal of Laboratory and Clinical Medicine·Pranee WinichagoonSuthat Fucharoen
Feb 23, 2011·International Journal of Laboratory Hematology·G FucharoenS Fucharoen
Dec 17, 2016·International Journal of Laboratory Hematology·H SrivorakunS Fucharoen
Apr 19, 2018·Clinical Chemistry and Laboratory Medicine : CCLM·Kritsada SinghaSupan Fucharoen
Dec 20, 2008·American Journal of Clinical Pathology·Zhaohai YangVishnu V B Reddy
Oct 22, 2011·The Korean journal of laboratory medicine·Ji-Eun KimJin-Yeong Han

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Methods Mentioned

BETA
PCR
electrophoresis

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