Evaluation of a new selective medium for the isolation of Corynebacterium urealyticum

Journal of Medical Microbiology
J ZapardielF Soriano

Abstract

A new selective medium (CBU agar) was compared with blood agar (BA) medium for primary isolation of Corynebacterium urealyticum from urine and skin samples of hospitalised patients. Overall, the CBU agar detected C. urealyticum in 14 (4.6%) of 302 urine samples and the BA medium detected the organism in four (1.3%), but most cultures which were positive only on CBU agar had < 10(4) cfu/ml. Six strains of C. urealyticum were isolated from 60 skin samples with CBU agar, whereas none was detected with BA. Although most skin samples had heavy inocula, the selective agar facilitated the recognition of low colony counts (< or = 10 cfu/plate) of C. urealyticum by reducing the growth of competing flora. Challenge of the selective medium with reference and clinical strains showed that CBU agar was inhibitory for gram-negative bacteria and reduced the gram-positive flora, allowing the growth of C. urealyticum strains. The new selective medium appears to be a useful epidemiological tool to study urinary and skin colonisation by C. urealyticum.

Citations

Jul 26, 2002·The Journal of Small Animal Practice·M L SuarezG Santamarina
Feb 25, 2014·Journal of Nephrology·Laura CappuccinoRoberto Pontremoli
Jun 28, 2014·Journal of Microbiological Methods·Osamu TsuzukibashiMasatomo Hirasawa
Nov 6, 2007·La Presse médicale·Paul Meria, Paul Jungers
Jun 19, 2008·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·F Soriano, A Tauch
Mar 30, 2018·Journal of Clinical Pathology·Sally Cheuk Ying WongKwok-Yung Yuen
Feb 9, 2021·Frontiers in Medicine·Els Van de PerreEmmanuel Letavernier

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