Evaluation of a novel automated volumetric flow cytometer for absolute CD4+ T lymphocyte quantitation

Cytometry. Part B, Clinical Cytometry
Morgane GossezGuillaume Monneret

Abstract

Bead-based single platform cytometry technology (SPT) is the gold standard when performing CD4 absolute counting. However, it presents drawbacks as precision depends on various critical steps (for example, pipetting methodology, overtime stability of beads, stability of fluidics, regular recalibration…) and thus requires skilled operators. The fully automated volumetric SPT AQUIOS CL (Beckman Coulter) has recently emerged as an alternative with no need of beads. It may help improving results standardisation and fulfilling requirements for certification (ISO 15189). In this study, we assessed SPT AQUIOS CL performances in accordance to requirements for ISO 15189 accreditation. We evaluated repeatability and reproducibility (precision), bias (trueness), uncertainty (total error), range limits (linearity, quantification, detection limits), and inter-reagent/inter-sample contaminations in enumerating CD4+ T-cells. Concomitantly, we compared AQUIOS CL CD4+ T-cell values with the results obtained with our routine bead-based SPT (that is, FC500 Beckman Coulter, bead-based SPT), on blood samples from 148 patients representative of clinical laboratory routine workload. Every result (repeatability, reproducibility, trueness, total error)...Continue Reading

References

Jan 5, 2002·Clinical Chemistry and Laboratory Medicine : CCLM·J Kristiansen
Dec 30, 2003·Cytometry. Part B, Clinical Cytometry·Ian StorieDavid Barnett
Apr 27, 2005·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Tandakha Ndiaye DieyeLuc Kestens
Apr 5, 2013·Cytometry. Part B, Clinical Cytometry·Ulrich SackClaude Lambert
Sep 12, 2013·Cytometry. Part B, Clinical Cytometry·Shabnam TangriUNKNOWN ICSH/ICCS Working Group
Sep 12, 2013·Cytometry. Part B, Clinical Cytometry·Brent WoodUNKNOWN ICSH/ICCS Working Group
Nov 26, 2013·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Ulrich Sack, Attila Tárnok

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Citations

Aug 25, 2016·Critical Care : the Official Journal of the Critical Care Forum·Rens ZonneveldFrans B Plötz
Jan 22, 2017·Cytometry. Part B, Clinical Cytometry·Valentina GrossiMariangela Manfredi
Mar 16, 2017·Cytometry. Part B, Clinical Cytometry·Lesley E ScottWendy S Stevens
Oct 28, 2017·Cytometry. Part B, Clinical Cytometry·Luc Kestens, Frank Mandy
Feb 22, 2018·Clinical Chemistry and Laboratory Medicine : CCLM·Simon DegandtXavier Bossuyt
Nov 19, 2019·Cytometry. Part B, Clinical Cytometry·Francis Mandy
Sep 1, 2017·Intensive Care Medicine Experimental·Mehdi ZouiouichGuillaume Monneret
Dec 14, 2018·Cytometry. Part B, Clinical Cytometry·Michel TicchioniUNKNOWN Association Française de Cytométrie accreditation working group (AFC-AG)
Apr 14, 2021·Critical Care : the Official Journal of the Critical Care Forum·Fabienne VenetUNKNOWN RICO study group
Oct 13, 2021·Journal of Clinical Laboratory Analysis·Lin SunWei Guo

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