Evaluation of a novel biphasic culture medium for recovery of mycobacteria: a multi-center study.

PloS One
Zhenling CuiZhongyi Hu

Abstract

Mycobacterial culture and identification provide a definitive diagnosis of TB. Culture on Löwenstein-Jensen (L-J) medium is invariably delayed because of the slow growth of M. tuberculosis on L-J slants. Automated liquid culture systems are expensive. A low-cost culturing medium capable of rapidly indicating the presence of mycobacteria is needed. The aim of this study was to develop and evaluate a novel biphasic culture medium for the recovery of mycobacteria from clinical sputum specimens from suspected pulmonary tuberculosis patients. The biphasic medium consisted of 7 ml units of L-J slant medium, 3 ml units of liquid culture medium, growth indicator and a mixture of antimicrobial agents. The decontamination sediments of sputum specimens were incubated in the biphasic culture medium at 37°C. Mycobacterial growth was determined based on the appearance of red granule sediments and the examination using acid-fast bacilli (AFB). The clinical sputum specimens were cultured in the biphasic medium, on L-J slants and in the Bactec MGIT 960 culture system. Among smear-positive specimens, the mycobacteria recovery rate of the biphasic medium was higher than that of the L-J slants (P<0.001) and similar to that of MGIT 960 (P>0.05). Am...Continue Reading

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Citations

Apr 25, 2014·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·F RageadeA van Belkum
Feb 9, 2016·Journal of Microbiological Methods·Seon Ah CheonTae Jung Park
Nov 19, 2015·Frontiers in Microbiology·Shady Asmar, Michel Drancourt
Mar 13, 2018·Laboratory Medicine·HebatAllah Abd El Monaem BassiounyAmira Ezzat Khamis Amine

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