Abstract
Turbidity tests are commonly used for screening blood units for the presence of sickle cell trait (SCT) before transfusion to specific patient populations, based on recommendations of the AABB. In this pilot study, we evaluate a new method for screening blood donors and blood units for the presence of sickle hemoglobin. This study was based at King Abdulaziz University Hospital in Jeddah, Saudi Arabia. Study participants were approached consecutively between July 24, 2016, and August 8, 2016. Blood donors, control individuals, and known patients with sickle cell disease (SCD) were tested using both a point-of-care testing technology (Sickle SCAN, Biomedomics, Inc.) and hemoglobin capillary electrophoresis (HEP). Corresponding blood units were also tested using Sickle SCAN. A total of 200 donors, 13 blood units, and 57 patients and controls were included. Sensitivity and specificity of Sickle SCAN for detection of SCT and SCD was 100%, when compared to HEP as the gold standard. Sickle SCAN is a rapid test that shows high sensitivity and specificity for identification of hemoglobin S among blood donors and when used for testing blood units.
References
Jun 1, 1995·The Journal of Pediatrics·C H PegelowD Brambilla
Jun 22, 2000·The New England Journal of Medicine·E P VichinskyE A Manci
Jul 27, 2006·Annals of Saudi Medicine·Zaki NasserullahManal Al Wabel
Jun 19, 2007·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Nasser Abdulrahman AlhamdanAbdul Jamil Choudhry
Feb 23, 2013·Lab on a Chip·Xiaoxi YangSergey S Shevkoplyas
Apr 1, 2015·Blood·Adetola A KassimMichael R DeBaun
Sep 18, 2015·BMC Medicine·Julie KanterXiaoxi Yang
Oct 21, 2015·BMC Research Notes·Samuel Antwi-BaffourDavid Nana Adjei
May 6, 2016·Saudi Medical Journal·Salwa I HindawiFatima Azher
Aug 16, 2016·EBioMedicine·David O Osei-HwediehMark T Gladwin
Nov 20, 2016·British Journal of Haematology·Helen V NewUNKNOWN British Committee for Standards in Haematology
Feb 6, 2017·Lancet·Russell E WareMiguel R Abboud
Aug 29, 2017·Blood Cells, Molecules & Diseases·Patrick T McGann, Carolyn Hoppe
Nov 18, 2017·Annals of Hematology·Luke R SmartPatrick T McGann
Sep 22, 2018·BMC Hematology·Akueté Yvon SegbenaValériane Leroy
May 1, 2017·The Journal of Applied Laboratory Medicine·Joesph R Wiencek, Garrett S Booth