Evaluation of a prototype DNA probe test for the noncultural diagnosis of gonorrhea.

Journal of Clinical Microbiology
P A Granato, M R Franz

Abstract

A prototype, nonisotopic, chemiluminescent DNA probe test called the Gen-Probe PACE (Probe Assay-Chemiluminescence Enhanced) system for Neisseria gonorrhoeae (Gen-Probe, San Diego, Calif.) was compared with conventional Martin-Lewis culture medium in JEMBEC plates for the laboratory diagnosis of gonorrhea. This 2-h noncultural assay is based upon the use of an acridinium ester-labeled DNA probe. The rRNA-directed DNA probe hybridizes with the target rRNA, and the hybridized probe is separated from the unhybridized probe through the use of magnetic microparticles. The esterified acridinium is hydrolyzed from the hybridized probe by the addition of an alkaline hydrogen peroxide solution, resulting in the production of visible light which is measured in a luminometer. The amount of light generated is directly proportional to the amount of gonococcal target rRNA present in the sample. A total of 407 clinical specimens (203 urethral and 204 endocervical) were collected from high-risk walk-in patients attending a sexually transmitted disease clinic. Separate patient specimens were collected for culture on Martin-Lewis medium in JEMBEC plates and for DNA probe assay. Statistical analysis of the overall comparative results showed that ...Continue Reading

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Citations

Jun 1, 1995·Molecular Biotechnology·P G IsaacC M Clee
Aug 25, 2005·Journal of General Internal Medicine·Phyllis L CarrJoseph S Pliskin
Apr 16, 2016·Indian Journal of Medical Microbiology·R Verma, S Sood
Jun 1, 1990·Journal of Clinical Microbiology·F C TenoverI Nachamkin
May 1, 1990·Journal of Clinical Microbiology·R RossauH Van Heuverswyn
Dec 1, 1995·Journal of Clinical Microbiology·S ChingJ Zenilman

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