PMID: 6106550May 1, 1980Paper

Evaluation of a radioreceptor assay for beta-adrenoceptor antagonists in plasma

European Journal of Clinical Pharmacology
D B BarnettS R Nahorski

Abstract

A radioreceptor assay (RRA) recently developed in this laboratory for beta-adrenoceptor antagonists in plasma was evaluated in normal volunteers and compared with a radioimmunoassay (RIA) for propranolol. The RRA depends upon the ability of beta-adrenoceptor antagonists to complete with a radiolabelled ligand for beta-adrenoceptor binding sites on lung membranes. Unlike other assays, it measures biologically active drugs including active metabolites of the parent compound. In volunteers given a single oral dose of (+/-)-propranolol, considerable differences between the two assay methods were demonstrated. In other experiments this difference was shown to relate to the RIA's sensitivity to the inactive (+)-isomer of propranolol and possibly to inactive metabolites. The facility of the RRA in measuring plasma levels of several other non-selective beta-adrenoceptor antagonists was also demonstrated. By employing (-)-propranolol as the standard in the RRA, all of these drugs can be directly compared with a single and relatively simple assay technique.

References

Jan 1, 1976·Clinical Pharmacokinetics·G Johnsson, C G Regàrdh
Dec 1, 1978·European Journal of Pharmacology·S R NahorskiD B Barnett
Oct 1, 1978·European Journal of Pharmacology·S R Nahorski
Nov 27, 1978·European Journal of Clinical Pharmacology·A P Douglas-JonesA Lee
Mar 1, 1979·Clinical Pharmacokinetics·P A Routledge, D G Shand
Oct 1, 1978·British Journal of Clinical Pharmacology·A J WoodD G Shand
Oct 1, 1977·Journal of Pharmaceutical Sciences·R E Kates, C L Jones
Jan 1, 1970·Clinical Pharmacology and Therapeutics·D G ShandJ A Oates

❮ Previous
Next ❯

Citations

Aug 29, 1980·European Journal of Pharmacology·K Sriwatanakul, S R Nahorski
Apr 1, 1986·Clinical Biochemistry·J W HubbardK K Midha
Jan 1, 1985·Journal of Ocular Pharmacology·T KailaR Huupponen
Mar 1, 1991·Journal of Pharmaceutical Sciences·T Kaila
Nov 1, 1984·Ophthalmology·M S PassoE M Van Buskirk
Apr 1, 1988·Scandinavian Journal of Dental Research·K LaurikainenP Vilja
Feb 1, 1983·British Journal of Clinical Pharmacology·N DesaiD B Barnett
Feb 1, 1987·Acta Ophthalmologica·R HuupponenA Urtti
Jun 1, 1994·Journal of Pharmaceutical and Biomedical Analysis·J SmisterováR A De Zeeuw

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.