Evaluation of a rapid ELISA technique for detection of circulating antigens of Toxoplasma gondii

Microbiology and Immunology
Rui ChenCuie Fu

Abstract

To evaluate a modified rapid ELISA method for detecting CAg during Toxoplasma gondii infection, we analyzed the specificity and sensitivity of the ELISA method by using experimental Toxoplasma infection in rabbits and also tested this method in human samples including 5428 serum, 548 cerebrospinal fluid and two breast milk samples. We prepared PcAb, and used it for rapid one-step sandwich ELISA testing in which an incubation time in the regular ELISA procedure was omitted. This method detected CAg at the concentration of 31.2 ng/mL, and no cross-reaction was found with antigens of protozoa (Cryptosporidium parvum, Plasmodium falciparum), trematode (Schistosoma japonicum, Paragonimus sp.) and nematode (Brugia malayi, Ancylostoma duodenale, Ascaris lumbricoides and Trichinella spiralis). CAg was detected in rabbit serum 3 days after infection, and optical density values reached a peak 9-13 days after infection, then declined gradually. Among human serum samples, the positive rate of CAg was 2.11% in cerebral paralysis patients, whereas it was 0.22% or 0.71% in patients without neurological symptoms or in uncomplicated pregnant women. The difference among these three groups was statistically significant (P < 0.05). The positive ra...Continue Reading

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Citations

Apr 30, 2013·The Lancet Infectious Diseases·Cedric P YansouniMarleen Boelaert
Apr 22, 2011·The Journal of Parasitology·Mu-Xin ChenRen-Li Zhang
Apr 15, 2010·Journal of Immunoassay & Immunochemistry·O EmeliaN Rahmah
May 29, 2012·Revista Do Instituto De Medicina Tropical De São Paulo·Susana A Zevallos LescanoPedro Paulo Chieffi
Dec 8, 2009·The Korean Journal of Parasitology·Juan Hua QuanYoung-Ha Lee
Mar 21, 2017·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·S PishkariM Mohebali
Jul 9, 2020·International Journal of Environmental Research and Public Health·Mohammed Nasiru WanaRoslaini Abd Majid
Oct 11, 2017·Frontiers in Microbiology·Ming PanBang Shen
Aug 14, 2019·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Abeer M AbdalhamedRaafat M Shaapan
Jun 9, 2020·BMC Public Health·Solomon Ngutor Karshima, Magdalene Nguvan Karshima
Feb 2, 2018·Food Science & Nutrition·Reza Zolfaghari EmamehSeppo Parkkila

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