Evaluation of a rapid immunochromatographic test strip for detection of Rabies virus in dog saliva samples

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Songsri KasempimolpornVisith Sitprija

Abstract

An immunochromatographic test strip for Rabies virus was evaluated with dog saliva samples. The test was initially validated against 237 dogs of known infection status, and then evaluated in the field with 1,290 live dogs. By validation of paired saliva-brain specimens obtained from dogs at necropsy, the saliva strip test was 94.4% specific and 93.0% sensitive when compared to the gold standard fluorescent antibody test (FAT) on brain smears. The sensitivity and specificity of a nested polymerase chain reaction (nPCR) assay using saliva were 100% compared to the FAT results. The performance of strip test with field saliva samples from street dogs had a specificity of 98.7% in comparison to nPCR as the reference method. As the strip test kit can potentially be used outside the laboratory and be applicable as an on-site testing assay, it represents a powerful screening tool for epidemiological surveys and disease control. The test could be useful for the surveillance of rabies in dogs and, in particular, be used to monitor the success of rabies control programs.

References

Nov 14, 1997·Journal of Clinical Microbiology·P R HeatonJ E Whitby
May 23, 1998·Journal of Clinical Microbiology·P CrepinH Bourhy
Jun 20, 1998·Annals of Internal Medicine·D L NoahJ E Childs
Aug 2, 2000·Journal of Clinical Microbiology·S KasempimolpornV Sitprija
Aug 2, 1965·JAMA : the Journal of the American Medical Association·J B VAUGHNK W NEWELL
Apr 27, 2004·Microbiology and Immunology·Songsri KasempimolpornVisith Sitprija
Mar 1, 2006·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·T NagarajV Ravi
Dec 5, 2006·Preventive Veterinary Medicine·S KasempimolpornV Sitprija
Jul 14, 2007·Journal of Virological Methods·BoKyu KangDaeSub Song
Apr 23, 2008·Microbiology and Immunology·Akira NishizonoKinjiro Morimoto

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Citations

Dec 19, 2013·TheScientificWorldJournal·Reeta Subramaniam Mani, Shampur Narayan Madhusudana
Jun 2, 2017·PLoS Neglected Tropical Diseases·Bounlom DouangngeunStuart D Blacksell
Nov 6, 2020·PLoS Neglected Tropical Diseases·Kazunori KimitsukiAkira Nishizono
Feb 6, 2021·Scientific Reports·Martin FayeOusmane Faye
May 1, 2021·Comparative Immunology, Microbiology and Infectious Diseases·J M NaleS A Ingle

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Methods Mentioned

BETA
nucleic acid amplification
PCR

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