Evaluation of a real-time method of simultaneous amplification and testing in diagnosis of Mycoplasma pneumoniae infection in children with pneumonia

PloS One
Wei LiShiqiang Shang

Abstract

Mycoplasma pneumoniae (M. pneumoniae) infection can cause community acquired pneumonia in children. A real-time method of simultaneous amplification and testing of M. pneumoniae (SAT-MP) was developed to diagnose M. pneumoniae targeting a region of the ribosomal RNA. The SAT-MP assay can accurately identify M. pneumoniae with a detection range from 101 to 107 CFU/ml. In this study, the specimens from 315 children with pneumonia were collected and analyzed by SAT-MP in parallel with real-time PCR method and IgM ELISA assay. The positive rates of these specimens examined by SAT-MP assay, real-time PCR method and IgM ELISA assay were 16.51%, 15.56% and 12.70% respectively. While there was statistical significance (p = 0.04) between SAT-MP assay and IgM ELISA assay, no statistical significance (p = 0.25) was found between SAT-MP assay and real-time PCR method and these two methods had high consistency (kappa value = 0.97). These findings indicate that the newly developed SAT-MP assay is a rapid, sensitive and specific method for identifying M. pneumoniae with potential clinical application in the early diagnosis of M. pneumoniae infection.

References

Apr 2, 2003·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·F DaxboeckC Wenisch
Jun 8, 2010·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·K LoensM Ieven
Oct 19, 2011·Thorax·Michael HarrisUNKNOWN British Thoracic Society Standards of Care Committee
Mar 30, 2013·Diagnostic Microbiology and Infectious Disease·Laurent BussonOlivier Vandenberg
Sep 18, 2013·Diagnostic Microbiology and Infectious Disease·Bryan H SchmittRobin Patel
Dec 19, 2013·The Pediatric Infectious Disease Journal·T Prescott Atkinson, Ken B Waites
Sep 12, 2014·Journal of Clinical Microbiology·Roger Dumke, Enno Jacobs
Jun 11, 2016·Journal of Virological Methods·Xuping WuYongfeng Yang

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Citations

Mar 3, 2020·The Pediatric Infectious Disease Journal·Angela Rocio CopeteLázaro Agustín Vélez
May 31, 2018·World Journal of Microbiology & Biotechnology·Wenyuan ShiYanhua Zeng
Aug 3, 2021·Frontiers in Cellular and Infection Microbiology·Yan ZhengYuanyuan Zhang

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Methods Mentioned

BETA
PCR
ELISA
antigen assay

Software Mentioned

SAT
MP

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