PMID: 7517958May 1, 1994Paper

Evaluation of a safe sputum processing method for detecting tuberculosis

Journal of Clinical Pathology
A RattanR Kumar

Abstract

To evaluate a safe sputum processing method for detection of tuberculosis in developing countries. A sample processing method was developed in which acid fast bacilli were killed with 1% sodium hypochlorite and concentrated by flotation on a layer of xylene before staining by the Ziehl Neelsen or auramine O methods. Best results were obtained by auramine O staining after flotation. Staining by the Ziehl Neelsen method after flotation gave better results than direct Ziehl Neelsen staining without flotation. The flotation method with Ziehl Neelsen staining offers advantages for smear preparation in the tuberculosis control programmes of developing countries.

References

Jun 1, 1979·Tubercle·P P Contijo Filho, K S Fonseca
Aug 11, 1992·Annals of Internal Medicine·S W DooleyD E Snider
Jun 6, 1991·The New England Journal of Medicine·P F BarnesD E Snider
Sep 1, 1982·Tubercle·C H Collins
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Citations

Aug 20, 2010·Journal of Clinical Microbiology·Padmapriya P BanadaDavid Alland
Jan 1, 2008·Infection and Drug Resistance·Nora MorcilloJuan Carlos Palomino
Jan 15, 2014·Diagnostic Microbiology and Infectious Disease·Ponrut PhunpaeWatchara Kasinrerk
Jul 19, 2011·Tuberculosis·Sagarika HaldarJaya S Tyagi
Sep 30, 2006·The Lancet Infectious Diseases·Karen R SteingartMadhukar Pai

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