Evaluation of a series of silk fibroin protein-based nonwoven mats for use as an anti-adhesion patch for wound management in robotic surgery

Journal of Biomedical Materials Research. Part a
Yu-Chi WangMu-Hsien Yu

Abstract

A novel anti-adhesion nonwoven mat mainly composed of silk fibroin protein (SFP) was fabricated via the single-spinneret electrospinning technique. A series of SFP-based electrospun nonwoven mats containing additives of different synthetic polymer ratios, such as pure SFP, SFP/poly(vinyl alcohol) (PVA), SFP/polyethylene glycol (PEG), and SFP/polyethylene oxide (PEO) were produced and compared. All membranes were porous and had diameters of 324.02 ± 113.7, 308.86 ± 74.02, 366.22 ± 115.81, and 341.82 ± 119.42 nm, respectively. The average pore size for each membrane was 1.132 ± 0.99, 0.811 ± 0.424, 0.975 ± 0.741, and 0.784 ± 0.497 μm2 . No nonwoven mats showed significant cytotoxicity toward fibroblast cells based on the results of MTT assays. Surprisingly, for all groups of SFP-based nonwoven mats, nitrate formation was reduced by up to 94.55 ± 14.50%, 92.16 ± 19.38%, 91.28 ± 28.375%, and 92.00 ± 12.64% in lipopolysaccharide-induced RAW 264.7 macrophages model. Tissue anti-adhesion potential was evaluated in an in vitro fibroblast cell adhesion model and in vivo wounded mice model. In vitro, the mean cell anti-adhesion percentage of fibroblast cells changed over time in the following order: PVA/SFP > SFP > PEG/SFP∼PEO/SFP. In vi...Continue Reading

References

Jun 1, 2000·Human Reproduction·B W HellebrekersJ B Trimbos
Jul 21, 2004·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Christine WeisDorothee Freytag
Apr 1, 2005·The Heart Surgery Forum·Sabine H DaebritzJörg S Sachweh
Sep 13, 2005·The Spine Journal : Official Journal of the North American Spine Society·Tsuyoshi KatoKenichi Shinomiya
Oct 30, 2007·Journal of Pharmaceutical Sciences·Joshua S BoatengGillian M Eccleston
Jul 14, 2010·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Kjell Olmarker
Apr 2, 2013·Annals of Plastic Surgery·Som KohanzadehJames N Long
May 21, 2013·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Daisuke NiwaShinji Takeoka
Nov 22, 2013·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Sang-Ho BaeByong-Taek Lee
May 16, 2014·BioMed Research International·Cornelia Erfurt-Berge, Regina Renner
Jan 1, 2013·Materials Science & Engineering. C, Materials for Biological Applications·Meng-Yi BaiHui-Ching Yang
Nov 6, 2015·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Wing P ChanMeng-Yi Bai
Feb 4, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Lin Zhu, Yu-Qing Zhang
Jun 11, 2016·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Jae Eok KoOh Hyeong Kwon

Citations

Jul 12, 2020·Materials·Heekyung ParkDonghyun Lee
Nov 4, 2019·Advanced Drug Delivery Reviews·Mazaher GholipourmalekabadiSubhas C Kundu

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

© 2021 Meta ULC. All rights reserved