Evaluation of a serodiagnostic test using Ascaris suum haemoglobin for the detection of roundworm infections in pig populations

Veterinary Parasitology
J VlaminckP Geldhof

Abstract

The significant economical consequences of infections with Ascaris suum in pigs are already well documented. However, due to the subclinical nature of the disease and the lack of practical diagnostic means, ascariasis often remains undiagnosed. Here we describe the development and evaluation of a novel indirect ELISA using the purified A. suum haemoglobin (AsHb) molecule as an antigen. Initial validation using sera from 190 pigs experimentally infected twice a week with A. suum and Trichuris suis (25 and 5 eggs kg(-1)day(-1) respectively) demonstrated that the AsHb ELISA is able to detect long-term exposure to A. suum with a high sensitivity and specificity (99.5% and 100.0% respectively). Furthermore, this serological technique proved to be more sensitive than faecal examination on week 7 and 14 of the experiment (99.5% and 100% compared to 59.5% and 68.4% respectively). Cross-reactivity caused by T. suis infection was shown to be limited after analysing sera from pigs with an experimental T. suis mono-infection. Seroconversion was shown to occur from week 6 onwards in pigs receiving 100 A. suum eggs 5 times a week. Preliminary testing of the ELISA on six randomly selected farms confirmed the results obtained in the artificial...Continue Reading

References

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Citations

Sep 7, 2013·PLoS Neglected Tropical Diseases·Natalie E NieuwenhuizenAndreas L Lopata
Nov 10, 2015·Current Tropical Medicine Reports·Poppy H L Lamberton, Peter M Jourdan
Nov 11, 2017·Transboundary and Emerging Diseases·J CharlierE Claerebout
Nov 27, 2019·The American Journal of Tropical Medicine and Hygiene·Sammy M NjengaJeffrey W Priest
Feb 20, 2020·Parasites & Vectors·Yue XieGuangyou Yang
Jan 22, 2021·Porcine Health Management·Emelie PetterssonPer Wallgren

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