PMID: 9177575May 30, 1997Paper

Evaluation of a simple PCR technique for the diagnosis of Trypanosoma vivax infection in the serum of cattle in comparison to parasitological techniques and antigen-enzyme-linked immuno sorbent assay

Acta Tropica
Marc Desquesnes

Abstract

Polymerase chain reaction (PCR) with specific oligonucleotides for the amplification of Trypanosoma vivax DNA has been developed by Masiga et al. (1992) to detect the presence of T. vivax DNA in biting flies. The aim of this experiment was to evaluate the efficacy of this technique when applied directly on cattle serum, without DNA purification, to detect infection. The sensitivity of this PCR technique was compared with parasitological techniques, namely haematocrit centrifuge technique (HCT) and buffy coat method (BCM), and with the antigen-enzyme-linked immunosorbent assay (Ag-ELISA) for T. vivax developed by Nantulya and Lindqvist (1989). Blood and serum samples were collected from four calves experimentally infected with a stock of T. vivax from French Guyana (IL4007). During the first 51 days of infection, a total of 164 samples were collected and processed using the four tests. Mean percentages of positive results were 68% with HCT, 59% with BCM, 4% with Ag-ELISA and 64% with PCR. Parasitological and PCR techniques yielded approximately the same sensitivities. PCR was able to detect active infection in serum samples when parasitaemia was over 10(3) trypanosomes/ml. With this isolate of T. vivax the Ag-ELISA was not found...Continue Reading

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Citations

Jan 25, 2011·Vector Borne and Zoonotic Diseases·Solange de Araujo MeloAna Lucia Abreu-Silva
Oct 6, 2001·Veterinary Parasitology·D FayeS Geerts
Mar 12, 2016·Parasites & Vectors·Samson LetaMelkamu Bezie
Jul 15, 2015·PLoS Neglected Tropical Diseases·Ahmadou H DickoJérémy Bouyer
Nov 21, 2012·PloS One·Soumaïla PagabeleguemJérémy Bouyer
Dec 19, 2012·Research in Veterinary Science·Michael I TakeetIkhide G Imumorin
May 23, 2009·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Satoru KonnaiKazuhiko Ohashi
Jul 27, 2012·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Katyane de Sousa AlmeidaAdjair Antonio do Nascimento
Mar 22, 2007·Veterinary Parasitology·J L GonzalesL M Siles
Mar 28, 2008·PLoS Neglected Tropical Diseases·Tim Stinear, Paul D R Johnson
Sep 3, 2010·Parasitology Research·Alfredo MijaresRamon Portillo
Jan 19, 2012·PLoS Neglected Tropical Diseases·Dorothy Yeboah-ManuGerd Pluschke
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May 6, 2009·Veterinary Parasitology·J B OliveiraA Urbina

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