Evaluation of a six-dye multiplex composed of 27 markers for forensic analysis and databasing.

Molecular Genetics & Genomic Medicine
Shuangshuang WangHaibo Luo

Abstract

Short tandem repeat (STR) markers play a significant role in genetic applications and have proved to be effective for the personal identification in forensic medicine. In this study, a six-dye multiplex composed of 23 autosomal STR loci (TH01, D3S1358, Penta D, D6S1043, D21S11, TPOX, D1S1656, D12S391, Penta E, D10S1248, D22S1045, D19S433, D8S1179, D2S1338, D2S441, D18S51, vWA, FGA, D16S539, CSF1PO, D13S317, D5S818, D7S820), one Y chromosome STR (DYS391), two internal quality control markers (Quality Sensor QS1 and QS2), and Amelogenin was evaluated. Evaluation studies, including PCR-based studies, sensitivity studies, species specificity studies, stability studies, DNA mixture studies, concordance studies, and precision evaluations were performed according to the guidelines of "Validation Guidelines for Forensic DNA Analysis Methods (2016)" by the Scientific Working Group on DNA Analysis Methods (SWGDAM). In addition, the forensic characteristics of 357 unrelated male samples from Han and Hui populations in China were investigated using 27 markers. Full STR profiles were obtained from different reaction volumes (5 ~ 25 μl), cycle numbers (28 ~ 34 cycles) and annealing temperatures (58 ~ 62°C). All STR profiles were obtained at ...Continue Reading

References

Feb 11, 1988·Nucleic Acids Research·S A MillerH F Polesky
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Oct 29, 2004·Nature Reviews. Genetics·Mark A Jobling, Peter Gill
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Oct 3, 2018·Medicine, Science, and the Law·Marek KowalczykAndrzej Jakubczak
Nov 13, 2019·International Journal of Legal Medicine·Mingkun XieHaibo Luo

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Citations

Sep 23, 2021·International Journal of Legal Medicine·Bowei JiangLagabaiyila Zha

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Methods Mentioned

BETA
genotyping
PCR
electrophoresis

Software Mentioned

Modified
Power states
GeneMapper ID

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