Evaluation of a solid-phase test for erythrocyte antibody screening of pregnant women, patients and blood donors

Vox Sanguinis
S SallanderM Aqvist

Abstract

We have studied a previously described solid-phase test (SPH) for screening and identification of antibodies directed against erythrocyte antigens, in large sample numbers and throughout routine processing. The screening results of all samples from pregnant women, patients and blood donors (n = 36,701) sent to our blood bank from August 1992 to July 1993 were compiled and evaluated. Pregnant women were screened by the SPH-antiglobulin technique (IAT) and the SPH-enzyme-enhanced technique (ENZ), while the patients and blood donors were screened only by the SPH-IAT. Samples with known alloimmunization were excluded from this study. Positive screening reactions were further investigated by the SPH-IAT and SPH-ENZ techniques, the manual hemagglutination tests low ionic-strength solution IAT and 2-stage papain. Pregnant women: A positive reaction in one or both of the SPH tests was found in 1.2% of the samples. The SPH tests identified 99 (98.0%) and the manual methods 79 (78.2%) of a total of 101 antibodies. Of the identified antibodies, 61 were to antigens in the Rh blood group system, including 34 Rh immune globulin. The remainder had specificity directed against antigens K, Jka, M, S, Le(a) and Le(b). A positive reaction in the ...Continue Reading

References

Jul 1, 1976·Transfusion·H C Moore, P L Mollison
Nov 1, 1976·Transfusion·J Knopp
Jan 1, 1992·Vox Sanguinis·W J JuddC E Nugent
Oct 1, 1986·British Journal of Obstetrics and Gynaecology·P J BowellC C Entwistle
Apr 1, 1993·Transfusion·P D IssittJ Bumgarner

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Citations

Oct 19, 2001·Transfusion·B DiedrichA Shanwell
Aug 3, 2010·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Akihiro TakeshitaHitoshi Ohto
Sep 14, 2012·Hematology·Sangeeta PahujaManjula Jain
Apr 27, 2016·Hematology/oncology Clinics of North America·Jeanne E Hendrickson, Christopher A Tormey

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