Evaluation of a Streptococcus thermophilus strain with innate anti-inflammatory properties as a vehicle for IL-10 cDNA delivery in an acute colitis model

Cytokine
Silvina Del CarmenJean G LeBlanc

Abstract

The aim of this work was to develop a Streptococcus (S.) thermophilus strain with improved anti-inflammatory properties due to the incorporation of the therapeutic cDNA delivery plasmid pValac::il-10. To achieve this purpose, cells of S. thermophilus CRL807, previously selected as being an important anti-inflammatory strain, were electroporated with pValac::il-10 plasmid. In order to confirm the functionality of the developed strain, it was co-cultured with human epithelial cells Caco-2 and the production of IL-10 was evaluated by ELISA. Bacterial suspensions of S. thermophilus CRL807 containing pValac::il-10 plasmid or of the wild-type (WT) strain were administered in vivo using a murine model of intestinal inflammation. The animals treated with S. thermophilus CRL807 pValac::il-10 showed a lower body weight loss, microbial translocation to liver and damage scores in their intestines at macroscopical and microscopic levels. Furthermore, a significant increase was observed in the concentration of IL-10 in the intestinal contents of these mice compared to the rest of the experimental groups, accompanied by decreased levels of pro-inflammatory cytokines. The insertion of the therapeutic pValac::il-10 plasmid increased the intrins...Continue Reading

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Citations

Oct 13, 2015·Inflammatory Bowel Diseases·M Nedim InceMichael J Wannemuehler
Jul 30, 2016·Journal of Medical Microbiology·Alejandra de Moreno de LeBlancJean Guy LeBlanc
Jul 23, 2020·Applied Microbiology and Biotechnology·Noura S DosokySean S Davies
Jan 23, 2021·International Journal of Molecular Sciences·Ryszard PlutaStanisław J Czuczwar

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