Evaluation of a surface-sampling probe electrospray mass spectrometry system for the analysis of surface-deposited and affinity-captured proteins

Rapid Communications in Mass Spectrometry : RCM
Gary J Van BerkelStephen J Kennel

Abstract

A combined self-aspirating electrospray emitter/surfacing-sampling probe coupled with an ion trap mass spectrometer was used to sample and mass analyze proteins from surfaces. The sampling probe mass spectrometer system was used to sample and detect lysozyme that had been deposited onto a glass slide using a piezoelectric spotter or murine gamma-interferon affinity captured on a glass slide using surface-immobilized anti-gamma-interferon antibody. The detection level for surface-deposited lysozyme (spot size < or =200 microm) was approximately 1.0 fmol (approximately 100 fmol/mm2) as determined from the ability to measure accurately the protein molecular mass from the mass spectrum acquired by sampling the deposit. These detection limits may be sufficient for certain applications in which protein fractions from a separation method are collected onto a surface. Radiolabeled proteins were used to quantify the surface density of immobilized antibody and the efficiency of capture of the gamma-interferon on glass and higher surface area ceramic supports. The capture density of gamma-interferon at surface saturation ranged from about 23 to 50 fmol/mm2 depending on the capture surface. Nonetheless, mass spectrometric detection of affi...Continue Reading

References

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Citations

Sep 21, 2013·Journal of the American Society for Mass Spectrometry·Wei RaoDavid A Barrett
Jan 22, 2013·Annual Review of Physical Chemistry·Abraham K Badu-TawiahR Graham Cooks
Mar 7, 2014·Analytical Chemistry·A Gareth Brenton, A Ruth Godfrey
Oct 23, 2015·Journal of the American Society for Mass Spectrometry·Wei RaoZhibo Yang
Aug 18, 2009·Journal of Mass Spectrometry : JMS·Lee Chuin ChenKenzo Hiraoka
Nov 15, 2006·Journal of Mass Spectrometry : JMS
Aug 2, 2008·Journal of Mass Spectrometry : JMS·Gary J Van BerkelOlga Ovchinnikova
May 16, 2013·Journal of Mass Spectrometry : JMS·Kevin Aart Douglass, Andre R Venter
Sep 8, 2011·Mass Spectrometry Reviews·Zhong-Ping Yao
Dec 19, 2009·Journal of Mass Spectrometry : JMS·Vilmos Kertesz, Gary J Van Berkel
Sep 1, 2011·Food Additives & Contaminants. Part B, Surveillance·Elsa Reis Vasco, Paula Cristina Alvito
May 22, 2019·Natural Product Reports·Nicholas H OberliesHuzefa A Raja
Jul 18, 2009·Analytical Chemistry·Gary J Van BerkelRichard C King
Oct 21, 2020·Journal of the American Society for Mass Spectrometry·Haidy MetwallyRichard Oleschuk

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