Evaluation of a whole-blood WBC-reduction filter that saves platelets: in vitro studies

Transfusion
S LarssonDragica Paunovic

Abstract

In this study, a new WBC-reduction in-line filter that removes WBCs but not platelets was evaluated. Three WBC-reduced blood components were prepared: RBCs, plasma, and platelet concentrates (PCs). Whole-blood components (n = 30) were filtered within 2 to 4 hours after collection and then were centrifuged and separated into RBCs, plasma, and WBC-reduced buffy coat. Saline-adenine-glucose-mannitol solution was added to the RBCS: The WBC-reduced buffy coats were stored overnight; on the following day, PCs were prepared from pooled WBC-reduced buffy coats and stored in a medium composed of approximately 35 percent CPD plasma and 65 percent platelet additive solution (T-Sol, Baxter). The WBC-reduction capacity of the filter, the recovery of cells after filtration, and the in vitro storage of RBCs (n = 10) and platelets (n = 6) were evaluated. Mean and maximum WBC counts after filtration were 0.08 x 10(6) and 0.3 x 10(6), respectively, per filtered whole-blood unit. Recovery of RBCs (mean values) after filtration was 90 percent in whole-blood components and 73 percent in RBCS: Recovery of platelets (mean values) was 81 percent after filtration and 66 percent in PCS: The in vitro storage study of RBCs showed results comparable with p...Continue Reading

References

Nov 1, 1984·Transfusion·D B FrewinR W Beal
Jun 6, 2000·Vox Sanguinis·H GullikssonA Shanwell

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Citations

Jun 3, 1999·Transfusion Science·R N PieterszM J Seghatchian
Nov 27, 2001·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·B G Solheim, F Wesenberg
Jul 1, 2004·Transfusion·Karin JanetzkoHermann Eichler
Jul 22, 2004·Transfusion·Dragica PaunovicJoachim Riggert
Oct 6, 2006·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Shizue Hayashi Natori, Keisuke Kurita
Feb 14, 2006·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Shizue Hayashi NatoriAkon Higuchi
Aug 13, 2011·Transfusion·Philip C SpinellaJohn B Holcomb
Apr 12, 2003·Vojnosanitetski pregled. Military-medical and pharmaceutical review·Bela Balint
Jul 11, 2003·Vojnosanitetski pregled. Military-medical and pharmaceutical review·Bela Balint

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