Evaluation of agonal artifacts in the myocardium using a combination of histological stains and immunohistochemistry

The American Journal of Forensic Medicine and Pathology
E Edston

Abstract

The problem of discrimination between agonal artifacts and intravital ischemic myocardial lesions was studied with four histochemical stains [hematoxylin-eosin, Mallory's phosphotungstic acid hematoxylin (PTAH), modified luxol fast blue, and Lie's hematoxylin basic fuchsin picric acid (HBFP)] and with immunohistochemistry using two antibodies (antimyoglobin and anti-C5b-9). Seventy-five forensic autopsy cases were divided into six groups designed to represent successively shorter periods of agonal myocardial ischemia: (a) sudden deaths with coronary artery disease, macroscopically visible myocardial infarction, and/or fresh coronary thrombus; (b) unexplained sudden deaths without coronary artery disease; (c) accidental CO poisoning; (d) suicidal CO poisoning in cars; (e) suicidal hangings; and (f) instant traumatic deaths, i.e., total brainstem laceration or rupture of the thoracic aorta. From each heart, five pieces were removed from standardized locations, and six parallel sections were stained with each method (i.e., 30 sections from each heart). Hematoxylin-eosin and anti-C5b-9 were only positive in the first three groups, thus indicating specificity for intravital necrotic changes. The other staining methods were "positive...Continue Reading

References

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Citations

Sep 18, 2010·International Journal of Legal Medicine·Tony FracassoAndreas Schmeling
Jul 21, 2009·Forensic Science, Medicine, and Pathology·Neil E I Langlois
May 14, 2014·International Journal of Legal Medicine·Felix MayerStefanie Ritz-Timme
Dec 1, 2009·Pathology, Research and Practice·Annette M MayUrsus-Nikolaus Riede
Dec 29, 1998·Human Pathology·C J SmithB M Wagner
Jun 4, 2008·The American Journal of Forensic Medicine and Pathology·Carlo P CampobassoMassimo F Colonna
Jan 25, 2018·International Journal of Legal Medicine·Aleksandra AljaknaSara Sabatasso
Apr 16, 2002·The American Journal of Forensic Medicine and Pathology·Alfredo Ribeiro-SilvaMarcos A Rossi
Jan 27, 2007·Annales de pathologie·Céline Guilbeau-FrugierMarie Bernadette Delisle

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