Evaluation of allelic alterations in short tandem repeats in different kinds of solid tumors--possible pitfalls in forensic casework

Forensic Science International : Synergy
Micaela PoetschBritta Kleist

Abstract

Archival pathology specimens are nowadays a frequently used source in forensic identification or paternity testing, if no other material is available. A greater part of this archived material, however, consists of solid tumors known for aberrations in coding and non-coding regions of the genome. Therefore, alterations of short tandem repeats (STRs) used in forensic casework are also possible. In our study of 118 solid tumors, 46 lymph node metastases, and 16 distant metastases with the AmpFlSTR trade mark Profiler Plus PCR amplification kit comprising nine STR loci, we detected four kinds of changes between normal and tumor tissue: partial loss of one allele (pLOH), complete loss of one allele (LOH), occurrence of an additional allele and occurrence of a new allele instead of that found in normal tissue. Twenty-two percent of the tumor lesions displayed pLOH, but only in 14% one allele was completely lost. New alleles could be demonstrated in 18% of tumors, and in 8% the new allele in the tumor tissue replaced the one found in normal tissue. The changes were distributed over all nine STRs, but the STRs mostly affected were FGA, D3S1558, D18S51 and D21S11. The occurrence of new alleles in the tetra-nucleotide repeats correlated ...Continue Reading

References

Sep 1, 1992·Forensic Science International : Synergy·A J JeffreysA Sonnberg
Feb 1, 1994·Nature Genetics·P GillK Sullivan
Jun 19, 1998·American Journal of Human Genetics·B BrinkmannB Rolf
Jun 5, 1999·Forensic Science International : Synergy·J A ThomsonP G Debenham
Apr 12, 2001·Cancer Genetics and Cytogenetics·M PoetschC Woenckhaus
Jun 8, 2001·Forensic Science International : Synergy·P Hoff-OlsenB Olaisen
Jul 27, 2001·Melanoma Research·A RichettaS Calvieri
Jan 5, 2002·International Journal of Legal Medicine·J A ThomsonP G Debenham
Feb 21, 2002·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Francesca OrlandiFranco Maria Venanzi
May 12, 2010·Forensic Science International. Genetics·Toni M Diegoli, Michael D Coble

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Citations

Feb 26, 2009·International Journal of Legal Medicine·Vincent CastellaPatrice Mangin
Feb 26, 2008·Genetic Testing·Susi PelottiAlberto Cicognani
May 8, 2009·Folia Histochemica Et Cytobiologica·Witold PepińskiJerzy Janica
May 30, 2009·Croatian Medical Journal·Zoran BudimlijaMechthild Prinz
Oct 24, 2014·Proceedings. Biological Sciences·Helen M BrowningJohn A Hammond
Feb 5, 2010·Forensic Science International. Genetics·Kristin DuvalChantal J Frégeau
Mar 20, 2009·Clinica Chimica Acta; International Journal of Clinical Chemistry·Venkanna NarkutiLakshmi N Mangamoori
Mar 29, 2006·Journal of Forensic Sciences·John M Butler
May 13, 2008·Journal of Forensic Sciences·Anke JungeBurkhard Madea
Feb 10, 2010·International Journal of Cancer. Journal International Du Cancer·Amanda Capes-DavisR Ian Freshney
Nov 9, 2012·International Journal of Cancer. Journal International Du Cancer·Amanda Capes-DavisLiz Kerrigan
Feb 12, 2020·Molecular Genetics & Genomic Medicine·Zhen DangYequan Wang
Jun 24, 2011·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Sara JordanBradley J Monk
Mar 5, 2016·International Journal of Molecular Medicine·Ilaria GiustiVincenza Dolo
Nov 29, 2020·Forensic Science International. Genetics·Pamela TozzoLuciana Caenazzo

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