Evaluation of an alternative S100b assay for use in cardiac surgery: relationship with microemboli and neuropsychological outcome

Perfusion
Donald WhitakerStanton P Newman

Abstract

The aim of the study was to investigate the relationship between S100b release, neuropsychological outcome and cerebral microemboli. Peri-operative assay of the astroglial cell protein S100b has been used as a marker of cerebral damage after cardiac surgery but potential assay cross-reactivity has limited its specificity. The present study uses an alternative enzyme-linked immunoabsorbant assay (ELISA) for serum S100b that has documented sensitivity and specificity data in patients undergoing coronary artery bypass grafting (CABG). Fifty-five consecutive patients undergoing routine CABG surgery received serial venous S100b sampling at five time points: i) Pre-operative, ii) At the end of cardiopulmonary bypass (CPB), iii) 6 hrs, iv) 24 hrs and v) 48 hrs post skin closure. A previously described sandwich ELISA with monoclonal anti- S100b was used. This assay has a lower limit of detection of 0.04 microg/L and < 0.006% reactivity with S100a at a concentration of 100 microg/L S100a. Cerebral microemboli during surgery were recorded by transcranial Doppler monitor over the right middle cerebral artery. Evidence of cerebral impairment was obtained by comparing patients' performance in a neuropsychological battery of 9 tests administ...Continue Reading

References

Jan 1, 1995·Brain Research Bulletin·D B ZimmerW Song
Jun 23, 1997·Journal of Immunological Methods·A J GreenE J Thompson
Mar 20, 1998·Stroke; a Journal of Cerebral Circulation·E B RingelsteinM Siebler
May 7, 1999·Stroke; a Journal of Cerebral Circulation·S Manzar
Jul 3, 1999·The Annals of Thoracic Surgery·R E AndersonJ Vaage
Sep 2, 1999·Stroke; a Journal of Cerebral Circulation·S KilminsterD W Holt
Oct 20, 1999·Heart·S Newman, J Stygall
Dec 29, 1999·The Journal of Thoracic and Cardiovascular Surgery·S WestabyP W Halligan
Dec 29, 1999·The Journal of Thoracic and Cardiovascular Surgery·D GeorgiadisT Deufel
Sep 19, 2000·British Journal of Anaesthesia·M S AliR Vaughan
Mar 28, 2001·The Annals of Thoracic Surgery·L SteedS P Newman
Nov 2, 2001·The Journal of Thoracic and Cardiovascular Surgery·J Vaage, R Anderson
Mar 21, 2002·JAMA : the Journal of the American Medical Association·Diederik Van DijkUNKNOWN Octopus Study Group
Mar 21, 2003·The Annals of Thoracic Surgery·Donald Whitaker
Aug 12, 2003·Brain Research Bulletin·A PetzoldE J Thompson
Dec 4, 2003·Health Psychology : Official Journal of the Division of Health Psychology, American Psychological Association·Jan StygallMichael J Harrison
Jan 30, 2004·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Donald C WhitakerRobin K Walesby
Jun 30, 2004·The Annals of Thoracic Surgery·Vincent FazioDamir Janigro

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Citations

Nov 9, 2011·British Journal of Anaesthesia·J P CataE Farag
Jun 25, 2008·Methods : a Companion to Methods in Enzymology·Jaclyn A FreudenbergHongtao Zhang
May 7, 2013·International Journal of Cardiology·Shi-Min Yuan
Jan 20, 2016·Seminars in Cardiothoracic and Vascular Anesthesia·Nikil PatelEmma M L Chung

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