Evaluation of an automated culture system for detecting bacterial contamination of platelets: an analysis with 15 contaminating organisms

Transfusion
M E BrecherL Stutzman

Abstract

Approximately 1 in 2000 platelet components are bacterially contaminated. The time to detection of 15 seeded organisms in platelets recovered from an automated culture system was studied. Isolates of Bacillus cereus, Bacillus subtilis, Candida albicans, Clostridium perfringens, Corynebacterium species, Enterobacter cloacae, Escherichia coli, Klebsiella oxytoca, Propionibacterium acnes, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Serratia marcescens, Streptococcus pyogenes, and Streptococcus viridans were inoculated into Day 2 apheresis platelet components to obtain a final concentration of approximately 10 and 100 CFU per mL (2 units/organism). Each bag was sampled 10 times (20 mL/sample). Four mL of each sample was inoculated into standard aerobic and anaerobic bottles and into aerobic and anaerobic bottles containing charcoal; 2 mL was inoculated into pediatric aerobic bottles (so as to maintain a 1:10 ratio of sample to media) and 1 mL into thioglycollate broth. With the exception of P. acnes, all organisms were detected in a mean of 9.2 to 25.6 hours. A range of 10 serial dilutions in inoculating concentrations was associated with an overall 10.1-percent difference in detection time. A mean of...Continue Reading

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Citations

Feb 9, 2002·Journal of Clinical Apheresis·N D Depcik-SmithM E Brecher
Jun 1, 2005·Anesthesiology Clinics of North America·Lawrence T Goodnough
Dec 23, 2003·Transfusion Medicine Reviews·Morris A BlajchmanFederico Baeza
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Aug 5, 2005·Transfusion·Sandra Ramirez-Arcos, Mindy Goldman
Sep 26, 2003·Transfusion·Jay S Epstein, Jaroslav G Vostal

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