Evaluation of an automated instrument for viability and concentration measurements of cryopreserved hematopoietic cells

Laboratory Hematology : Official Publication of the International Society for Laboratory Hematology
S E SzaboK Loper

Abstract

Two important parameters for determination of deleterious effects of cellular processing on hematopoietic progenitor cells are cell viability and concentration. The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins Hospital evaluated the Beckman Coulter Vi-Cell automated instrument for the measurement of these two parameters. Using 33 thawed hematopoietic progenitor cell samples, automated Vi-Cell viability results were compared to those obtained using the standard trypan blue manual method. In addition, cell concentrations from these samples were compared with results from the Model Z2 Coulter Counter. Chinese Hamster Ovary cells were used for the evaluation of Vi-Cell linearity at the Beckman Coulter Cellular Analysis Development Center. Significant correlation was obtained when the two methods were compared for both cell concentration and percentage viability (P < .0001). The results of the linearity study indicated that the Vi-Cell is linear from approximately 5 x 10(4) to greater than 1 x 10(7) cells/mL. The Vi-Cell uses sample volumes as low as 0.5 mL; cell diameters may be 2 to 70 microns. The Vi-Cell automated instrument offers many significant advantages for cell analyses in today's busy laboratory environment.

Citations

Oct 21, 2010·Journal of Industrial Microbiology & Biotechnology·Leo L ChanBo Lin
Apr 6, 2013·Journal of Industrial Microbiology & Biotechnology·Daniel J LavertyLeo Li-Ying Chan
Jun 22, 2012·Journal of Fluorescence·Leo L ChanNing Lai
Feb 15, 2012·Antimicrobial Agents and Chemotherapy·Kabilan VelliyagounderDaniel H Fine
Dec 14, 2011·Journal of Visualized Experiments : JoVE·Elizabeth J AbrahamSusan Qian
Feb 23, 2010·Biochemical and Biophysical Research Communications·James A G CrispoT C Tai
Sep 11, 2007·International Journal of Laboratory Hematology·L PengL Bai
Mar 26, 2011·Journal of Clinical Laboratory Analysis·Jang Su KimJun Koon Chang
Nov 10, 2011·International Endodontic Journal·X HuangD Pei
May 24, 2012·Biochemical and Biophysical Research Communications·Karin Åström-OlssonLars Grip
Nov 13, 2013·Biological Procedures Online·Dongpyo HongMoongu Jeon
Apr 5, 2011·Optometry and Vision Science : Official Publication of the American Academy of Optometry·Maureen BoostPauline Cho
Dec 1, 2011·Optometry and Vision Science : Official Publication of the American Academy of Optometry·Maureen V BoostPauline Cho

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

Related Papers

Journal of Clinical Pathology
R Biggs, R L Macmillan
Apoptosis : an International Journal on Programmed Cell Death
Leo Li-Ying ChanBo Lin
Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Leo Li-Ying ChanBo Lin
Clinical and Diagnostic Laboratory Immunology
A WeinbergK Lamb
© 2022 Meta ULC. All rights reserved