Evaluation of an automated liquid-handling system (Tecan Genesis RSP 100) in the Abbott LCx assay for Chlamydia trachomatis

Journal of Clinical Microbiology
K L Hanson, C P Cartwright

Abstract

The present study investigated the feasibility of automating the specimen-pipetting component of sample preparation in the LCx Chlamydia assay (LCx-CT assay; Abbott Laboratories, Chicago, Ill.) by using a commercially available liquid-handling system (Tecan Genesis RSP100; Tecan Inc., Research Triangle Park, N.C.). The Tecan instrument proved to be comparable in both precision and accuracy to a manual multipipettor (Eppendorf model 4850; Eppendorf Scientific, Westbury, N.Y.). The Tecan instrument was extensively checked for evidence of specimen-to-specimen transfer, and no level of contamination sufficient to generate a signal above the background in the LCx-CT assay was detected. Finally, pipetting speed was significantly improved by using the Tecan instrument. A mean time of 2.5 min was required to pipette a complete LCx-CT assay carousel (20 samples and 4 controls) with the Tecan instrument, whereas 8.4 min was required to pipette a comparable number of samples manually (P < 0.001).

Citations

Jul 22, 2008·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A Hadgu, M Sternberg
Mar 24, 2004·Drug Discovery Today·Alaa-Eldin F Nassar, Rasmy E Talaat
Aug 16, 2002·Sexually Transmitted Infections·M A Chernesky
Apr 6, 2006·The Journal of Infectious Diseases·Alula Hadgu
Nov 2, 2015·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Adarsh RamakumarPataje G S Prasanna
May 7, 2010·Lab on a Chip·Jenifer Clausell-TormosChristoph A Merten

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