PMID: 9164061Apr 1, 1997Paper

Evaluation of an avidin-biotin-peroxidase method with a monoclonal antibody to type IV collagen in the differential diagnosis of bullous pemphigoid and epidermolysis bullosa acquisita

The Journal of Dermatology
M Bowszyc-DmochowskaT Nishikawa

Abstract

There are reports in which an immunohistochemical technique with a monoclonal antibody to type IV collagen has been employed for differentiating between bullous pemphigoid (BP) and epidermolysis bullosa acquisita (EBA). The aim of this study was to determine whether this method could be used routinely. Biopsies (paraffin-embedded lesional skin containing a blister) from currently diagnosed patients with clinical features suggesting BP or EBA were examined by an avidin-biotin-peroxidase (ABC) technique. Sera were tested by indirect immunofluorescence on salt-split skin (IF) and immunoblotting (IB). In all cases which exhibited clear type IV collagen staining, the results of the ABC technique agreed with results of both IF and IB. In one confirmed EBA case, it was impossible to unequivocally localize type IV collagen, because it stained very faintly. Taking into consideration the results of our study, data indicating that the level of blistering might not coincide with the localization of immunoreactants in EBA cases and the possibility of an enzymatic destruction of lamina densa, we conclude that the ABC method is unsuitable for differentiation between BP and EBA.

References

Sep 1, 1992·The Journal of Investigative Dermatology·G J GiudiceL A Diaz
Aug 1, 1992·The British Journal of Dermatology·S MiyagawaT Shirai
Jul 1, 1992·Journal of the American Academy of Dermatology·W R GammonR A Briggaman
Jun 1, 1991·The Journal of Investigative Dermatology·L J MeyerJ J Zone
Jun 1, 1994·The Journal of Investigative Dermatology·G J GiudiceL A Diaz
Oct 1, 1995·The British Journal of Dermatology·S H WakelinF Wojnarowska
Mar 1, 1994·Clinical and Experimental Dermatology·G Kirtschig, F Wojnarowska
Dec 1, 1993·Journal of Dermatological Science·M DmochowskiT Nishikawa
Nov 15, 1993·The Journal of Immunology : Official Journal of the American Association of Immunologists·G J GiudiceL A Diaz
Oct 1, 1993·Journal of the American Academy of Dermatology·E Fonseca, F Contreras

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Citations

Sep 6, 2007·The Journal of Small Animal Practice·P B HillT Olivry
Nov 11, 2005·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Christian RoseDetlef Zillikens

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