Evaluation of an enterocin AS-48 enriched bioactive powder obtained by spray drying

Food Microbiology
S AnanouEva Valdivia

Abstract

Enterocin AS-48 is a cationic cyclic bacteriocin produced by Enterococcus faecalis with broad bactericidal activity. Currently we are assaying the efficacy of AS-48 as biopreservative in foods. In this work we have applied the spray drying process to different AS-48 liquid samples to obtain active dried preparations. We have also assayed different methods, heat, UV irradiation and filtration, to inactivate/remove the AS-48 producer cells from the samples. Best results were obtained for the sample from CM-25 cation exchange, for which it was also possible to completely eliminate/inactivate the producer cells by heat or UV irradiation without loss of activity. When added at 0.016% or 5% to Brain Heart Infusion broth or to skim milk, respectively, the AS-48 powder caused early and complete inactivation of Listeria monocytogenes. A partial inhibition of Staphylococcus aureus was achieved in broth and in skim milk supplemented with 2.5% and 10% AS-48 powder, respectively.

References

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Citations

Dec 11, 2014·International Journal of Molecular Sciences·María José Grande BurgosRosario Lucas
Feb 13, 2010·Current Opinion in Biotechnology·Antonio GálvezRosario Lucas
Jun 1, 2010·Probiotics and Antimicrobial Proteins·Hikmate AbriouelAntonio Gálvez
Jan 15, 2021·Comprehensive Reviews in Food Science and Food Safety·Yanlong CuiXin Lü

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