Evaluation of an enzyme immunoassay for detection of Chlamydia psittaci in vaginal secretions, placentas, and fetal tissues from aborting ewes

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
T P Sanderson, A A Andersen

Abstract

A commercially available enzyme immunoassay (EIA) for the detection of Chlamydia trachomatis in human urogenital and conjunctival specimens was compared with isolation in cell culture for the detection of Chlamydia psittaci in vaginal and placental swabs from aborting ewes and swabs of aborted fetal tissues. The EIA on vaginal swabs collected from 10 ewes experimentally infected with C. psittaci had a sensitivity of 85.7% and a specificity of 85.7%. Vaginal swabs collected at the time of abortion or within 3 days were the best samples for detection of chlamydial infection. The 29 vaginal swabs collected during this period from experimentally infected ewes were all strongly EIA-positive, and chlamydia were isolated from 28. The EIA on vaginal swabs from 78 field cases of abortion had a sensitivity of 78.0% and a specificity of 76.8%. The EIA on swabs of cotyledons from 65 placentas had a sensitivity of 100% and a specificity of 75.0% compared with isolation in cell culture. The EIA on 57 swabs of fetal tissues or body fluids from 10 aborted fetuses or weak lambs from experimentally infected ewes had a sensitivity of 26.6% and a specificity of 88.1% compared with isolation in cell culture. Limitations of the EIA are discussed.

References

Oct 27, 1979·The Veterinary Record·K A Linklater, D A Dyson
Jul 1, 1989·Diagnostic Microbiology and Infectious Disease·D K Stein, A M Sugar
Mar 1, 1986·Diagnostic Microbiology and Infectious Disease·J Schachter
Feb 1, 1986·Journal of Clinical Microbiology·L V HowardJ E Herrmann
Jun 1, 1986·Genitourinary Medicine·K C MohantyM H Hambling
Oct 1, 1986·European Journal of Clinical Microbiology·L GrillnerH Hammar
Oct 1, 1986·European Journal of Clinical Microbiology·P Pothier, A Kazmierczak
Jul 1, 1986·American Journal of Obstetrics and Gynecology·R B JonesR R Fisher
Aug 1, 1985·Journal of Periodontology·W J LoescheE C Morrison
Apr 3, 1986·The New England Journal of Medicine·P SaikkuA Nissinen
May 3, 1984·The New England Journal of Medicine·M R TamR C Nowinski
Jan 1, 1984·The American Journal of the Medical Sciences·M BlumD F Roses
Jan 1, 1984·Postgraduate Medical Journal·J T Hart
Mar 1, 1983·The British Veterinary Journal·F W Johnson
Mar 1, 1985·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·J Nagai, A J McAllister

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Citations

Aug 11, 2015·Veterinary Microbiology·A Rodolakis, K Laroucau
Nov 17, 2010·Acta Veterinaria Scandinavica·Bodil Ström HolstCatharina Linde Forsberg
Apr 27, 2007·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Giovanna MasalaSebastiana Tola
Apr 1, 1992·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·T P Sanderson, A A Andersen
Jul 1, 1993·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·C A Kirkbride
Jan 1, 1993·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·J C NietfeldD H Zeman

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