Evaluation of an enzyme immunoassay for diagnosis of bovine leptospirosis caused by Leptospira interrogans serovar hardjo type hardjo-bovis

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
G A TruebaC O Thoen

Abstract

Sensitivity and specificity of 4 different antigen preparations from Leptospira interrogans serovar hardjo were compared in an enzyme immunoassay for detection of antibodies against serovar hardjo type hardjo-bovis in serum. Two antigens prepared using detergents showed serogroup cross-reactivity. A mechanically extracted membrane and a lipopolysaccharide antigen showed a high degree of leptospiral serogroup specificity. The lipopolysaccharide antigen was the most suitable antigen for detection of anti-hardjo antibodies. Enzyme immunoassay was more sensitive than the microscopic agglutination test for detecting antibodies in serum from experimentally and naturally infected cattle. It was not possible to differentiate vaccinated from infected animals or to detect a secondary immune response in vaccinated animals that were subsequently infected.

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Citations

Jun 14, 2005·Veterinary Microbiology·Maria Rosa Quaresma BomfimMatilde Cota Koury
May 2, 2012·Irish Veterinary Journal·Eoin Gerard RyanMichael L Doherty
May 21, 2013·Journal of the American Veterinary Medical Association·Alicia D ZimmermanChristopher C L Chase
Jul 1, 1992·Journal of Bacteriology·G A TruebaR L Zuerner

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