PMID: 11906733Mar 22, 2002Paper

Evaluation of an enzyme-linked immunosorbent assay for detection of antibodies to Junin virus in rodents

Journal of Virological Methods
María A MoralesM Sabattini

Abstract

Junin virus is the etiological agent of Argentine hemorrhagic fever, a serious rodent-borne disease. An enzyme-linked immunosorbent assay (ELISA) to detect Junin virus IgG antibodies in rodents was evaluated using sera from 27 Calomys musculinus and five Calomys laucha, inoculated experimentally with a live attenuated strain of this arenavirus. The test performance was compared against an indirect immunofluorescence assay (IFA). The ELISA had a sensitivity and specificity of 100% and a reproducibility of 87.9% for samples with titers above the selected cut-off value. IFA had lower sensitivity (53%) with the same specificity. The ELISA results were similar, whether carried out on whole blood or serum samples, thus eliminating the need for serum separation. A high correlation (K=0.86) between ELISA and IFA results was obtained from 1011 wild sigmodontine and murine rodents collected within and outside of the Argentine hemorrhagic fever endemic area. These results indicate that Junin virus IgG ELISA is the most suitable assay for detection of Junin virus antibodies in rodent samples.

Citations

Nov 26, 2003·Journal of the American Academy of Dermatology·Omar Lupi, Stephen K Tyring
Apr 4, 2003·International Journal of Antimicrobial Agents·Cybele C GarcíaElsa B Damonte
Oct 27, 2015·Vector Borne and Zoonotic Diseases·Ivani BisordiBenedito A L da Fonseca
Sep 20, 2005·Critical Care Clinics·David C Pigott
Dec 2, 2008·Journal of Medical Virology·Agustín E UreVíctor Romanowski
Jan 1, 2015·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·R KerberJ ter Meulen

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Argentine Hemorrhagic Fever

Argentine hemorrhagic fever (AHF) is an endemo-epidemic disease caused by junín virus (JUNV), a member of the arenaviridae family. Discover the latest research on AHF here.

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