Evaluation of an enzyme-linked immunosorbent assay that uses ferrous metal beads for determination of antihistoplasmal immunoglobulins G and M.

Journal of Clinical Microbiology
S E ZimmermanL J Wheat

Abstract

A rapid enzyme-linked immunosorbent assay (ELISA) for the detection of human class-specific antibodies to Histoplasma capsulatum (histoplasmal immunoglobulin M [HIgM] and histoplasmal IgG [HIgG]) was developed by using antigen adsorbed onto polycarbonate-coated ferrous beads. In the ELISA method all the reagents used were commercially available. In 135 specimens from patients with confirmed histoplasmosis, sensitivities were 76% for complement fixation (CF), 53% for immunodiffusion (ID), and 64% for the ELISA for HIgM and HIgG combined. The ELISA detected histoplasmal antibody in 36% of the specimens with negative antibody titers by CF and 46% of the specimens with negative antibody titers by ID. The ELISA detected histoplasmal antibody in 27% of specimens that were negative by both CF and ID. When limited to specimens collected within 4 months of the onset of histoplasmosis symptoms, sensitivities were 82% for CF, 63% for ID, and 86% for ELISA for HIgG and HIgM combined. Within this group, ELISA detected histoplasmal antibody in 90% of the specimens that were negative by CF, 76% that were negative by ID, and 100% that were negative by both CF and ID. The specificity of the ELISA could not be fully addressed since sera from pat...Continue Reading

References

Jun 9, 1978·JAMA : the Journal of the American Medical Association·P B TerryG D Roberts
Mar 1, 1986·Journal of Clinical Microbiology·J WheatR P Tewari
Jul 1, 1983·The American Review of Respiratory Disease·L J WheatZ Brahmi
Jul 1, 1981·Medicine·R A GoodwinR M Des Prez
Mar 1, 1981·Annals of Internal Medicine·L J WheatJ L Biesecker

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Citations

Oct 20, 2006·Expert Opinion on Biological Therapy·L Joseph Wheat
May 20, 2004·Journal of Medical Microbiology·Allan Jefferson GuimarãesRosely Maria Zancopé-Oliveira
Aug 18, 2006·Transplant Infectious Disease : an Official Journal of the Transplantation Society·L J Wheat
Feb 26, 2016·BMC Infectious Diseases·Marcos de Abreu AlmeidaRosely Maria Zancopé-Oliveira
Feb 16, 2021·Applied Microbiology and Biotechnology·María Agustina ToscaniniMaría Luján Cuestas
Oct 8, 1997·Journal of Clinical Microbiology·B L GomezR J Hay
May 20, 2003·Infectious Disease Clinics of North America·L Joseph Wheat, Carol A Kauffman
Nov 29, 2005·Revista iberoamericana de micología·Beatriz Consuelo Quinet LeimannRosely Maria Zancopé-Oliveira

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