PMID: 9187036Apr 1, 1997Paper

Evaluation of an enzyme-linked immunosorbent assay (ELISA) for the serological diagnosis of sarcoptic mange in swine

Veterinary Parasitology
W HollandersSet Bornstein

Abstract

An enzyme-linked immunosorbent assay (ELISA) using an extract from Sarcoptes scabiei var. culpes as antigen was evaluated for its usefulness in monitoring antibodies to S. scabiei var. suis in naturally infected pigs. Five hundred and fifty-eight serum samples from certified Sarcoptes-free pigs (120 from weaned piglets, 218 from fatteners and 220 from adult sows) and 94 samples from certified Sarcoptes-infected pigs (35 from weaned piglets, 45 from fatteners and 14 from adult sows) were examined. The cut-off optical density (OD) values, considered to reflect the presence of antibodies to S. scabiei var. suis, were determined to be 0.059, 0.213 and 0.374 for weaners, fatteners and sows, respectively. The specificity of the ELISA was high (> or = 98%) for all age groups. Sensitivity was highest in the piglets (80%), followed by fatteners (78%) and sows (50%). These results indicate that an ELISA may be a simple and valuable alternative to monitor S. seabiei var. suis infections in naturally infected weaners and fatteners.

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Citations

Jan 15, 2005·Archives of Dermatological Research·N HaasE Schein
Oct 10, 2007·Veterinary Research Communications·R GaluppiM P Tampieri
Mar 24, 2000·Veterinary Parasitology·H M van der HeijdenW A Hunneman
Apr 13, 2007·Clinical Microbiology Reviews·Shelley F Walton, Bart J Currie
Aug 3, 2014·Veterinary Parasitology·Hayley M ToetR Mark Sandeman
Oct 25, 2011·Diagnostic Microbiology and Infectious Disease·Rama JayarajShelley F Walton
Jul 3, 2010·Preventive Veterinary Medicine·F Rodríguez-CadenasF A Rojo-Vázquez
Mar 16, 2012·Annals of the New York Academy of Sciences·Russell W CurrierBart J Currie
May 24, 2011·Veterinary Parasitology·Alvaro OleagaChristian Gortázar
Jun 22, 2017·Parasites & Vectors·Larry G Arlian, Marjorie S Morgan
Dec 21, 2019·Revista Do Instituto De Medicina Tropical De São Paulo·Jorlan FernandesElba Regina Sampaio de Lemos
Jan 13, 2009·Clinical Microbiology Reviews·Bruno GottsteinKarsten Nöckler
Dec 2, 2008·Journal of Medical Virology·Agustín E UreVíctor Romanowski
Jul 18, 2006·International Journal for Parasitology·M ZalunardoR M Sandeman

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