PMID: 9557294Apr 29, 1998Paper

Evaluation of an expanded two-ELISA approach for confirmation of reactive serum samples in an HIV-screening programme for pregnant women

Journal of Medical Virology
G J Van DoornumR A Coutinho

Abstract

Serum specimens were collected from 31,232 pregnant women in Amsterdam between 1988 and 1995 in a screening programme for human deficiency virus (HIV) infection. The sera of 56 (0.179%) women tested were confirmed as positive for HIV. A total of 67 sera reacted positive or borderline by the screening enzyme-linked immunosorbent assay (ELISA) and indeterminate or negative by HIV-1 Western blot; 42 of these specimens were available for evaluation of the strategy for diagnosis of HIV infection. A two-ELISA approach with the second ELISA based on a principle different from that of the screening ELISA, expanded with the use of a membrane immunoassay based on two synthetic peptides specific for HIV-1 gp41 and HIV-2 gp36 envelope proteins, was compared with the Western blot analysis. Indeterminate results were resolved with a nucleic acid sequence-based amplification assay (NASBA) for HIV-1 RNA and a strip immunoassay (SIA) for the simultaneous detection of antibodies to HIV-1 or HIV-2 and HIV-1 p24 antigen. Eleven samples were weakly or borderline positive by the screening test and gave indeterminate results by Western blot. The expanded two-ELISA approach designated these sera as HIV-negative, and confirmed negative by NASBA and the...Continue Reading

References

Mar 7, 1991·Nature·J Compton
Aug 1, 1991·The British Journal of Psychiatry : the Journal of Mental Science·R Gardner
Jan 25, 1990·The New England Journal of Medicine·J B JacksonS Y Kwok
Jun 1, 1994·European Journal of Epidemiology·P J BindelsR A Coutinho
Jul 20, 1996·Lancet·L Gürtler

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