PMID: 9165453May 1, 1997Paper

Evaluation of an extracorporeal membrane oxygenation system using a nonporous membrane oxygenator and a new method for heparin coating

Journal of Pediatric Surgery
Y KitanoY Tsuchida

Abstract

A new heparin binding method was applied to a miniature extracorporeal membrane oxygenation (ECMO) system with a nonporous membrane oxygenator (the priming volume, 45 mL; the membrane surface area, 0.4 m2; maximal flow rate, 2 L/min) that is resistant to plasma leakage. The authors evaluated the stability of the immobilized heparin in vitro and the feasibility of this system in animals. Samples of hollow fibers and tubing were rinsed at 40 degrees C for 4 days in normal saline, Ringer's lactate, and 1 mol/L NaCl solution. Heparin activities on hollow fibers after rinsing were 99 +/- 2.3% (mean +/- SD), 96 +/- 3.9%, and 93 +/- 2.0% of the control in each solution, while those of the tubing were 87 +/- 4.1%, 86 +/- 3.1%, and 76 +/- 8.6%, respectively. Veno-arterial ECMO using this heparin-coated system was performed on five beagles (8 to 12 kg) for 10 hours. Neither major thrombus formation nor plasma leakage was detected during the procedure in spite of a low flow rate (300 mL/min) and a reduced activated clotting time (mean, 128 seconds). Platelets decreased to 52% of the control (P < .01) at 1 hour, but no progressive decrease was seen thereafter. Antithrombin-III decreased (P < .01) and thrombin/antithrombin III complex incre...Continue Reading

References

Mar 1, 1991·Journal of Pediatric Surgery·G C WhittleseyM D Klein
Mar 1, 1973·Journal of Biomedical Materials Research·J W EberleW J Rea
Apr 1, 1982·Thrombosis Research·C D Ebert, S W Kim
Jan 1, 1993·Acta Anaesthesiologica Scandinavica·O FjalldalV Magnússon
Dec 6, 1963·Science·V L GOTTR C DUTTON

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Citations

Oct 4, 2003·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Kurt A DasseRichard J Gilbert
Aug 3, 2006·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Bruce F MonzykRichard J Gilbert

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