Evaluation of an immunochromatographic assay for the detection of anti-hepatitis A virus IgM.

Virology Journal
Hyeok-Jin LeeDoo-Sung Cheon

Abstract

Hepatitis A virus (HAV) is a causative agent of acute hepatitis, which is transmitted by person-to-person contact and via the faecal-oral route. Acute HAV infection is usually confirmed by anti-HAV IgM detection. In order to detect anti-HAV IgM in the serum of patients infected with HAV, we developed a rapid assay based on immunochromatography (ICA) and evaluated the sensitivity of this assay by comparing it with a commercial microparticle enzyme immunoassay (MEIA) that is widely used for serological diagnosis. The newly developed ICA showed 100% sensitivity and specificity when used to test 150 anti-HAV IgM-positive sera collected from infected patients and 75 negative sera from healthy subjects. Also, the sensitivity of ICA is about 10 times higher than MEIA used in this study by determining end point to detect independent on infected genotype of HAV. In addition, the ICA was able to detect 1 positive sample from among 50 sera from acute hepatitis patients that had tested negative for anti-HAV IgM using the MEIA. Conclusively, ICA for the detection of anti-HAV IgM will be very effective for rapid assay to apply clinical diagnosis and epidemiological investigation on epidemics due to the simplicity, rapidity and specificity.

References

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Citations

Nov 13, 2018·Gastroenterology Nursing : the Official Journal of the Society of Gastroenterology Nurses and Associates·Jose Melendez-RosadoWilliam C Palmer
Jan 22, 2019·Future Microbiology·Camilla R de Almeida RibeiroVanessa S de Paula
Apr 19, 2019·Journal of Clinical Microbiology·Muktha S NatrajanJesse J Waggoner
May 2, 2012·Asian Pacific Journal of Tropical Medicine·Piyathida PongsiriYong Poovorawan
Mar 16, 2016·The American Journal of Tropical Medicine and Hygiene·Barbara W JohnsonMichael A Drebot
Dec 7, 2016·The Journal of Infectious Diseases·Barbara W JohnsonChristin H Goodman
Feb 10, 2020·Clinical Journal of Gastroenterology·Yukihiro YoshimuraNatsuo Tachikawa

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Methods Mentioned

BETA
ELISA
biosensor
biosensors
genotyping

Software Mentioned

ICA
AxSYM HAVAB - M

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