Evaluation of an in-house TgSAG1 (P30) IgG ELISA for diagnosis of naturally acquired Toxoplasma gondii infection in pigs

Veterinary Parasitology
Lais PardiniMaria Cecilia Venturini

Abstract

Toxoplasma gondii is an apicomplexan protozoan parasite which is able to infect a large variety of warm-blooded animals. Raw or undercooked pork has been regarded as an important source of infection for humans. The aim of this study was to evaluate an in-house enzyme-linked immunosorbent assay to diagnose natural T. gondii infection in swine using native affinity chromatography-purified T. gondii surface protein-1 (TgSAG1-ELISA) as antigen, comparing its performance to that of indirect fluorescent antibody test (IFAT) and immunoblotting (IB). To obtain a panel of sera showing the evolution of the antibody response in the time course 12 pigs were experimentally inoculated intravenously (iv) with tachyzoites of the T. gondii strains RH (clonal type I), ME49 (clonal type II) and NED (clonal type III) and serologically monitored for a period of 11 weeks. Both IFAT and ELISA showed a similar time course of antibody response to T. gondii; but by IFAT this response was characterized by rapidly rising titers with peaks at two weeks post inoculation (wpi), while the ELISA indices increased slowly and reached a maximum in most animals at five wpi. Three-hundred randomly selected sera from a total of 602 pigs of different ages derived fro...Continue Reading

References

Jun 3, 1988·Science·J A Swets
Dec 1, 1995·The Journal of Infectious Diseases·D K Howe, L D Sibley
Aug 1, 1993·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·U GrossJ Heesemann
Nov 27, 1999·International Journal for Parasitology·G JungersenP Lind
Jun 23, 2000·The Journal of Parasitology·H R GambleS F Parmley
Jul 7, 2001·Comparative Immunology, Microbiology and Infectious Diseases·M CondeJ González
Sep 22, 2001·International Journal for Parasitology·C LekutisJ C Boothroyd
Aug 25, 2004·Investigative Ophthalmology & Visual Science·Aize KijlstraTitia Kortbeek
Sep 11, 2004·The Journal of Parasitology·Geraldine M Saavedra, Ynés R Ortega
Jan 18, 2006·Veterinary Parasitology·Franz J Conraths, Gereon Schares
Aug 13, 2008·International Journal for Parasitology·Aize Kijlstra, Erik Jongert
Jun 30, 2009·Veterinary Parasitology·J P Dubey
Jul 14, 2010·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Sérgio Santos de AzevedoSolange Maria Gennari
Aug 10, 2016·Jornal de pediatria·Jaqueline Dario CapobiangoEdna Maria Vissoci Reiche

❮ Previous
Next ❯

Citations

Oct 11, 2014·Parasites & Vectors·Yanhua WangDelin Zhang
Oct 5, 2013·Experimental Parasitology·Yanhua WangMeng Wang
Nov 30, 2019·Journal of Feline Medicine and Surgery·María F GarcíaMaría A Stornelli
Dec 29, 2020·International Journal for Parasitology·Nora SchreiberPeter Deplazes

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibodies: Agglutination

Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here.