PMID: 1209161Nov 1, 1975Paper

Evaluation of an in vitro assay of lecithin:cholesterol acyl transfer rate in plasma

Scandinavian Journal of Clinical and Laboratory Investigation
L Wallentin, O Vikrot

Abstract

Crucial and previously criticized points in a slightly modified version of the Stokke-Norum assay of fractional lecithin:cholesterol acyl transfer (LACT) rate in plasma were studied. LCAT activity in the albumin added to the assay medium and negative influence of remaining organic solvent were important sources of error that it was necessary to eliminate. The assumption of equilibration of labeled cholesterol among lipoproteins in vitro was supported experimentally. Addition of isolated chylomicrons had no influence on initial LACT rate. Incubation time was decreased to 20 minutes to obtain a better estimate of initial LCAT rate in normals. Using gas-liquid chromatography to determine unesterified cholesterol concentration, the precision of measurement of molar LCAT rate was 5.2 per cent (coefficient of variation). Molar LCAT rate in healthy males 20-60 years of age was 56-130 mumol-1(-1)-h-1 (mean +/- 2 S.D.) and compared well with reports from other laboratories and in vivo measurements. At present this methodology is considered to be suitable for studies of LCAT and cholesterol turnover in different metabolic situations and clinical materials.

Citations

Oct 9, 1980·Clinica Chimica Acta; International Journal of Clinical Chemistry·H Dieplinger, G M Kostner
Jan 1, 1979·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M YamamotoM Sugano
Apr 1, 1984·European Journal of Clinical Investigation·A AlyL Wallentin
Dec 1, 1982·Clinical Biochemistry·J FrohlichK Hon
Jun 1, 1992·Scandinavian Journal of Clinical and Laboratory Investigation·H von SchenckJ Larsson
Jan 1, 1984·Toxicologic Pathology·P PaniE Laconi

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