Evaluation of an indwelling ruminal probe methodology and effect of grain level on diurnal pH variation in dairy cattle

Journal of Dairy Science
J E NocekW P Kautz

Abstract

To evaluate a ruminal probe for recording diurnal variation in rumen pH, we fitted three ruminally fistulated cows with probes affixed to the inside of the cannula. Probes were connected to a data logger and readings were recorded hourly. The experiment was a Latin square design with cows fed three different diets: 50, 60, and 70% grain as a total mixed rations once daily. Feed offerings and refusals were recorded daily. The experimental period was 21 d. The first 6 d were for adaptation, followed by 5-d rotations through each of the diets. Daily probe readings were recorded at 4-, 6-, 8-, 12-, and 24-h intervals. At each interval, readings were recorded (precleaned), a sample of rumen fluid was taken, and pH was measured in the laboratory. As probes were removed from the rumen, probe ends were cleaned with 0.1 N HCl and reinserted into the rumen, and a reading was recorded (postcleaned). No protective pH probe shields were used in this experiment. There were no differences between pre- and postcleaned pH readings across cows for all diets. Mean time under pH 5.0, 5.5, and 6.0 were 0.2, 2, and 7.2 h, respectively. Diet affected length of time under a certain pH, only for readings under pH 6. Diurnal pH profiles were monophasic ...Continue Reading

References

May 1, 1997·Journal of Dairy Science·J E Nocek

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Citations

Dec 28, 2018·Journal of Animal Physiology and Animal Nutrition·Huzur Derya ArikMustafa Selcuk Alatas
Aug 19, 2015·Animal Science Journal = Nihon Chikusan Gakkaihō·Shigeru Sato
Sep 26, 2014·Journal of Animal Science·W Z YangT A McAllister
Dec 1, 2020·Temperature : Multidisciplinary Biomedical Journal·Roberto Refinetti

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