Evaluation of an International Pharmacopoeia method for the analysis of indinavir sulfate by liquid chromatography

Journal of Pharmaceutical and Biomedical Analysis
Raja Satyanarayana YekkalaErwin Adams

Abstract

A gradient LC method for the determination of indinavir sulfate (IDV) and its impurities has been recently published in a consultation document of the International Pharmacopoeia, WHO Drug Information. The method uses a base-deactivated reversed-phase C18 column (25 cm x 4.6 mm i.d.), 5 microm kept at a temperature of 40 degrees C. The mobile phases consist of acetonitrile, phosphate buffer pH 7.5 and water. The flow rate is 1.0 ml/min. UV detection is performed at 220 nm. A system suitability test (SST) is described to govern the quality of the separation. The separation towards IDV components was investigated on 16 C18 columns and correlation was made with the column classification system developed in our laboratory. The method was evaluated using a Hypersil BDS C18 column (25 cm x 4.6 mm i.d.), 5 microm. A central composite design was applied to examine the robustness of the method. The method shows good precision, linearity, sensitivity and robustness. Six commercial samples were examined using this method.

References

Jun 5, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·A L JayewardeneJ G Gambertoglio
Mar 3, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·M L Foisy, J P Sommadossi
Mar 26, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Valerie A FrerichsGene D Morse
Apr 23, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Katharina M Rentsch
May 12, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Naser L RezkAngela D M Kashuba
Oct 26, 2005·Journal of Chromatography. a·Dóra ViskyErwin Adams

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Citations

Sep 11, 2007·Journal of Pharmaceutical Sciences·Raja YekkalaJos Hoogmartens
Dec 19, 2012·Journal of Pharmaceutical and Biomedical Analysis·R Nageswara RaoS Satyanarayana Raju
Apr 20, 2010·Journal of Pharmaceutical and Biomedical Analysis·Parul SinghA K Bakhshi
Sep 1, 2009·Journal of Pharmaceutical and Biomedical Analysis·Dunge AshenafiErwin Adams
Jun 3, 2008·Journal of Pharmaceutical and Biomedical Analysis·Murali PendelaErwin Adams
Oct 13, 2006·Journal of Pharmaceutical and Biomedical Analysis·Raja YekkalaErwin Adams
Sep 20, 2008·Journal of Pharmaceutical and Biomedical Analysis·Raja S YekkalaErwin Adams
Mar 23, 2007·Analytical and Bioanalytical Chemistry·Hans H Maurer
May 22, 2007·Analytical Chemistry·R K Gilpin, C S Gilpin

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