Evaluation of an NS1 antigen detection for diagnosis of acute dengue infection in patients with acute febrile illness

Diagnostic Microbiology and Infectious Disease
Keswadee LapphraVisanu Thamlikitkul

Abstract

Diagnosis of dengue infection during the febrile stage has been challenging. We evaluated the accuracy of NS1 protein detection in diagnosing dengue infection in patients presenting with acute febrile illness in Bangkok, Thailand. Of the 235 subjects presented with fever of unknown source within 5 days, 132 (56.2%) were male with the median age of 17.8 (range, 3-52) years. The median duration of fever was 4 (range, 1-5) days. One hundred seventy-one (72.8%) patients had dengue infection, of which 158 (92.4%) were secondary infections. The sensitivity of NS1 Ag test was 63.2% (95% confidence interval [CI], 55.7-70.0), and the specificity was 98.4% (95% CI, 91.7-99.7). The positive and negative predictive values were 99.0% and 52.5%, respectively. The immune complex dissociation by acid treatment increase sensitivity from 63.2% to 72%. In an endemic area, Plateliatrade mark NS1 Ag test has limited sensitivity but very high specificity for diagnosis of dengue infection in patients with acute febrile illness.

References

Aug 1, 1997·The Journal of Infectious Diseases·D W VaughnA Nisalak
Sep 6, 2000·Clinical and Diagnostic Laboratory Immunology·K ValdésM G Guzmán
Sep 6, 2003·Journal of Clinical Microbiology·Penelopie KorakaAlbert D M E Osterhaus
Jul 10, 2004·Clinical and Diagnostic Laboratory Immunology·Pei-Yun Shu, Jyh-Hsiung Huang
Mar 23, 2005·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·E M B LemesM A V Araujo
Apr 1, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Stuart D BlacksellNicholas P J Day
Sep 22, 2006·Clinical and Vaccine Immunology : CVI·Philippe DussartLaurence Baril

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Citations

Feb 23, 2013·Biosensors & Bioelectronics·Ana Carolina M S DiasRosa F Dutra
Nov 3, 2010·Transactions of the Royal Society of Tropical Medicine and Hygiene·Wanitda WatthanaworawitFrançois H Nosten
Apr 30, 2010·Vector Borne and Zoonotic Diseases·Kwoon-Yong PokLee-Ching Ng
Sep 17, 2009·The Pediatric Infectious Disease Journal·Sirichan ChunhakanAmpaiwan Chuansumrit
Aug 24, 2013·BMC Infectious Diseases·Boon-Teong TeohSazaly AbuBakar
Jul 14, 2010·PLoS Neglected Tropical Diseases·Monique da Rocha Queiroz LimaFlavia Barreto dos Santos
May 17, 2011·PLoS Neglected Tropical Diseases·Grace YapLee-Ching Ng
Aug 13, 2013·PLoS Neglected Tropical Diseases·Maria Rosario CapedingTram Anh Wartel
Sep 3, 2010·The American Journal of Tropical Medicine and Hygiene·Seok Mui Wang, Shamala Devi Sekaran
Sep 3, 2010·The American Journal of Tropical Medicine and Hygiene·Laurent ThomasRaymond Césaire
Nov 28, 2015·International Journal of Molecular Sciences·Yin-Liang TangHan-Chung Wu
Feb 1, 2011·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Chunya PuttikhuntWatchara Kasinrerk
Sep 8, 2009·Diagnostic Microbiology and Infectious Disease·Alvaro H RamirezFerdinando Liprandi
Feb 24, 2009·Diagnostic Microbiology and Infectious Disease·William John Hannan McBride
Feb 24, 2009·Diagnostic Microbiology and Infectious Disease·Hoang Lan PhuongPeter J de Vries
Feb 24, 2009·Protein Expression and Purification·Dipankar DasMavanur R Suresh
Jun 26, 2010·Journal of Medical Virology·Luiza A Castro-JorgeBenedito A L Fonseca
Apr 15, 2015·Journal of Virological Methods·Advaita GangulyHoon H Sunwoo
Jul 2, 2014·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Hao ZhangYuanping Zhou
Jan 20, 2015·Scientific Reports·Alessandra FigueiredoFrancisco E G Guimarães
Sep 18, 2014·Revista Do Instituto De Medicina Tropical De São Paulo·Pablo O A AcostaFelipe Gomes Naveca
Jan 29, 2014·The American Journal of Tropical Medicine and Hygiene·Séverine MatheusVincent Marechal
May 28, 2015·The American Journal of Tropical Medicine and Hygiene·Gustavo DayanFernando Noriega
Sep 5, 2013·Infection Ecology & Epidemiology·Anne Tuiskunen Bäck, Ake Lundkvist
Dec 28, 2017·Indian Journal of Critical Care Medicine : Peer-reviewed, Official Publication of Indian Society of Critical Care Medicine·Sanjay A MundheNikhil R Pawar
Apr 23, 2010·Clinical and Vaccine Immunology : CVI·Kovi BessoffElizabeth Hunsperger
Feb 6, 2009·Clinical and Vaccine Immunology : CVI·Pei-Yun ShuJyh-Hsiung Huang

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