Evaluation of anti-dengue activity of Carica papaya aqueous leaf extract and its role in platelet augmentation

Archives of Virology
Navita SharmaShashi Bala Singh

Abstract

Dengue disease is characterized by a marked decrease in platelet count, which is life threatening. In the present study, we investigated the antiviral activity of an aqueous extract of Carica papaya leaves (PLE) against dengue virus (DENV) and its effect on platelet augmentation. The anti-dengue activity of PLE in DENV-infected THP-1 cells was examined by immunoblotting and flow cytometry. The effect of PLE on erythrocyte damage was investigated using hemolytic and anti-hemolytic assays. Virus-infected THP-1 cells were assayed for IFN-α secretion. The effect of PLE on platelet augmentation in rats with cyclophosphamide-induced thrombocytopenia was also investigated. The platelet count of blood from the retro-orbital plexus of rats was determined on the 1st, 4th, 7th, 11th and 14th day of study. On the 14th day, the rats were sacrificed for histopathological examination of the liver, kidney and spleen. Plasma of thrombocytopenic rats was tested for thrombopoietin (TPO) and IL-6 secretion. The data suggest that PLE significantly decreases the expression of the envelope and NS1 proteins in DENV-infected THP-1 cells. A marked decrease in intracellular viral load upon PLE treatment confirmed its antiviral activity. This also resulte...Continue Reading

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Citations

May 6, 2020·Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences·Surya P SinghArbind Acharya
Sep 22, 2020·Ci Ji Yi Xue Za Zhi = Tzu-chi Medical Journal·Rashed Noor
Mar 12, 2021·Bioscience, Biotechnology, and Biochemistry·Yasuhide HiragaKota Kera
Mar 18, 2021·Revista Brasileira De Farmacognosia : Orgão Oficial Da Sociedade Brasileira De Farmacognosia·Kayode KomolafeAfolabi Akintunde Akindahunsi
May 1, 2021·Pathogens·Mohd Ridzuan Mohd Abd RazakAmi Fazlin Syed Mohamed
May 1, 2021·Pharmaceuticals·Tapan BehlDomenico Montesano
May 14, 2021·Frontiers in Pharmacology·Md Moklesur Rahman SarkerIsa Naina Mohamed
May 28, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·X Y LimT Y C Tan

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Methods Mentioned

BETA
electrophoresis
ELISA
blood collection
light microscopy
FACS

Software Mentioned

Cell Quest Pro
SPSS

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