Evaluation of antiangiogenic and antiproliferative potential of the organic extract of green algae Chlorella pyrenoidosa

Indian Journal of Pharmacology
Mahender KyadariThirumurthy Velpandian

Abstract

algae isolates obtained from fresh and marine resources could be one of the richest sources of novel bioactive secondary metabolites expected to have pharmaceutical significance for new drug development. This study was conducted to evaluate the antiangiogenic and antiproliferative activity of Chlorella pyrenoidosa in experimental models of angiogenesis and by MTT assay. lyophilized extract of C. pyrenoidosa was extracted using dichloromethane/methanol (2:1), concentrated and vacuum evaporated to obtain the dried extract. The crude extract was evaluated in the vascular endothelial growth factor (VEGF)-induced angiogenesis in in ovo chick chorioallantoic membrane assay (CAM) at various concentrations (n = 8) using thalidomide and normal saline as positive and untreated control groups, respectively. The crude extract was also subjected to the antiangiogenic activity in the silver nitrate/potassium nitrate cautery model of corneal neovascularization (CN) in rats where topical bevacizumab was used as a positive control. The vasculature was photographed and blood vessel density was quantified using Aphelion imaging software. The extract was also evaluated for its anti proliferative activity by microculture tetrazolium test (MTT) assa...Continue Reading

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Citations

Sep 15, 2016·Expert Review of Hematology·E Carlos Rodriguez-Merchan
Nov 2, 2015·Environmental Science and Pollution Research International·Patrick Y K YueEddy Y Zeng
Feb 16, 2021·Phytotherapy Research : PTR·Gönül Ulus

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Methods Mentioned

BETA
light photo microscopy
FCS
ELISA

Software Mentioned

Aphelion imaging

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