Evaluation of anticancer properties of a decoction containing Adenanthera pavonina L. and Thespesia populnea L

BMC Complementary and Alternative Medicine
Indeewari K S Lindamulage, Preethi Soysa

Abstract

A decoction composed of Adenanthera pavonina L. and Thespesia populnea L. is currently being used in the treatment of cancer patients. Lactate Dehydrogenase (LDH) release, (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) MTT, and Sulforhodamine B (SRB) assays were carried out to study cytotoxicity and anti-proliferative activity against the HEp-2 cells, 24 h post-treatment with the decoction. The mean (± SD) values of EC50 were 195.50 (±40.68), 120.02 (±29.82) and 77.06 (±8.80) μg/ml for LDH, MTT, and SRB assays respectively. These results strongly correlate the morphological changes observed in cells treated with the decoction. Induction of apoptosis was visualized by fluorescence microscopy stained with ethidium bromide/acridine orange dye mix. In addition, brine shrimp lethality assay showed an EC50 value at a higher concentration (1.96 mg/mL). These results suggest that the decoction prepared with Adenanthera pavonina L. and Thespesia populnea L. exhibits anti-proliferative activity and induces apoptosis on the HEp-2 cancer cells but no toxicity against Artemia salina.

References

Jan 1, 1992·Cancer Chemotherapy and Pharmacology·R C SteinR C Coombes
Apr 1, 1991·Annals of Medicine·S M Grundy
Jul 4, 1990·Journal of the National Cancer Institute·P SkehanM R Boyd
Apr 29, 1998·Cell Biology and Toxicology·C RenvoizéJ Bréard
Jun 25, 1999·Free Radical Biology & Medicine·J Johnson InbarajR Murugesan
Aug 17, 1999·Journal of Neuro-oncology·P BushunowT Coyle
Jun 1, 2000·Anti-cancer Drugs·M D ShelleyR G Fish
Nov 30, 2002·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Michael Le BlancJudith A Smith
Jul 22, 2004·Inflammopharmacology·Olumayokun A OlajideJanet M Makinde
May 12, 2005·BMC Biotechnology·Deborah RibbleYiqun G Shellman
Nov 1, 2005·Expert Opinion on Investigational Drugs·Kalliopi DodouPaul W Groundwater
Feb 21, 2006·Anesthesiology Clinics·Jacqueline Filshie, Carolyn N J Rubens
Jul 25, 2006·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·M Vasudevan, M Parle
Sep 5, 2006·Journal of Ethnopharmacology·Mani VasudevanMilind Parle
May 1, 1982·Planta medica·B N MeyerJ L McLaughlin
Jun 17, 2008·Journal of Natural Products·Sompong BoonsriAkkharawit Kanjana-Opas
Jul 23, 2011·Anais Da Academia Brasileira De Ciências·Paulo Michel P FerreiraAna F U Carvalho
Aug 28, 2012·Oriental Pharmacy and Experimental Medicine·Ramdas B PandhareShantaram G Khanage
Dec 3, 2013·Natural Product Research·R S MohammedD A Waly
Jul 31, 2014·BMC Complementary and Alternative Medicine·Preethi SoysaJayantha Wijayabandara

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Citations

Jul 9, 2016·BMC Complementary and Alternative Medicine·O E Kale, O Awodele

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Methods Mentioned

BETA
fluorescence microscopy
flow cytometry
MDA

Software Mentioned

Excel

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